Lung injury and recovery in a murine model of unilateral acid aspiration

Maria Amigoni, Giacomo Bellani, Margherita Scanziani, Serge Masson, Elisa Bertoli, Enrico Radaelli, Nicolò Patroniti, Alessandro D. Lelio, Antonio Pesenti, Roberto Latini

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Abstract

Background' Acid aspiration is a complication of general anesthesia. Most animal models developed to define Its patho-physiology have focused on the acute (≤24 h) phase of the Injury. The authors describe a model of acid aspiration allowing the study of this type of lung Injury over time. Methods: The authors instilled hydrochloric acid (0.1 M, 1.5 m1/kg) or normal saline In the right bronchus of mice. Lung Injury was evaluated at 6 h, 12 h, 24 h, and 2 weeks by assessing arterial blood gases, respiratory system compliance, lung wet weight normalized by body weight, lung myeloperoxidase activity, and histology. Twelve hours and 2 weeks after Injury, a computed tomography scan was obtained. Results: In the hydrochloric acid group, arterial oxygen tension decreased (P <0.05) at 12 and 24 h, whereas It recovered at 2 weeks; respiratory system compliance was lower both at 24 h and 2 weeks (P <0.05). Lung weight increased at 12 and 24 h (P <0.05). Myeloperoxidase activity peaked between 6 and 12 h. Computed tomography at 12 h showed that almost 30% of the Injured lung was abnormally aerated. Although reduced, the abnormalities were still present at 2 weeks as confirmed by a fibrotic scar well evident at histologic examination. Conclusion: The authors characterized a murine model of regional acid aspiration allowing long-term survival. Despite a partial recovery, at 2 weeks the Injury persisted, with evidence of fibrosis and lung compliance reduction. This long-term, low-mortality model seems suitable for assessment of the effects of different therapies on lung Injury and repair.

Original languageEnglish
Pages (from-to)1037-1046
Number of pages10
JournalAnesthesiology
Volume108
Issue number6
DOIs
Publication statusPublished - Jun 2008

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Lung Injury
Lung Compliance
Hydrochloric Acid
Respiratory System
Lung
Peroxidase
Acids
Wounds and Injuries
Tomography
Weights and Measures
Pulmonary Edema
Bronchi
General Anesthesia
Compliance
Cicatrix
Histology
Arterial Pressure
Fibrosis
Animal Models
Gases

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Amigoni, M., Bellani, G., Scanziani, M., Masson, S., Bertoli, E., Radaelli, E., ... Latini, R. (2008). Lung injury and recovery in a murine model of unilateral acid aspiration. Anesthesiology, 108(6), 1037-1046. https://doi.org/10.1097/ALN.0b013e318173f64f

Lung injury and recovery in a murine model of unilateral acid aspiration. / Amigoni, Maria; Bellani, Giacomo; Scanziani, Margherita; Masson, Serge; Bertoli, Elisa; Radaelli, Enrico; Patroniti, Nicolò; Lelio, Alessandro D.; Pesenti, Antonio; Latini, Roberto.

In: Anesthesiology, Vol. 108, No. 6, 06.2008, p. 1037-1046.

Research output: Contribution to journalArticle

Amigoni, M, Bellani, G, Scanziani, M, Masson, S, Bertoli, E, Radaelli, E, Patroniti, N, Lelio, AD, Pesenti, A & Latini, R 2008, 'Lung injury and recovery in a murine model of unilateral acid aspiration', Anesthesiology, vol. 108, no. 6, pp. 1037-1046. https://doi.org/10.1097/ALN.0b013e318173f64f
Amigoni M, Bellani G, Scanziani M, Masson S, Bertoli E, Radaelli E et al. Lung injury and recovery in a murine model of unilateral acid aspiration. Anesthesiology. 2008 Jun;108(6):1037-1046. https://doi.org/10.1097/ALN.0b013e318173f64f
Amigoni, Maria ; Bellani, Giacomo ; Scanziani, Margherita ; Masson, Serge ; Bertoli, Elisa ; Radaelli, Enrico ; Patroniti, Nicolò ; Lelio, Alessandro D. ; Pesenti, Antonio ; Latini, Roberto. / Lung injury and recovery in a murine model of unilateral acid aspiration. In: Anesthesiology. 2008 ; Vol. 108, No. 6. pp. 1037-1046.
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AU - Radaelli, Enrico

AU - Patroniti, Nicolò

AU - Lelio, Alessandro D.

AU - Pesenti, Antonio

AU - Latini, Roberto

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N2 - Background' Acid aspiration is a complication of general anesthesia. Most animal models developed to define Its patho-physiology have focused on the acute (≤24 h) phase of the Injury. The authors describe a model of acid aspiration allowing the study of this type of lung Injury over time. Methods: The authors instilled hydrochloric acid (0.1 M, 1.5 m1/kg) or normal saline In the right bronchus of mice. Lung Injury was evaluated at 6 h, 12 h, 24 h, and 2 weeks by assessing arterial blood gases, respiratory system compliance, lung wet weight normalized by body weight, lung myeloperoxidase activity, and histology. Twelve hours and 2 weeks after Injury, a computed tomography scan was obtained. Results: In the hydrochloric acid group, arterial oxygen tension decreased (P <0.05) at 12 and 24 h, whereas It recovered at 2 weeks; respiratory system compliance was lower both at 24 h and 2 weeks (P <0.05). Lung weight increased at 12 and 24 h (P <0.05). Myeloperoxidase activity peaked between 6 and 12 h. Computed tomography at 12 h showed that almost 30% of the Injured lung was abnormally aerated. Although reduced, the abnormalities were still present at 2 weeks as confirmed by a fibrotic scar well evident at histologic examination. Conclusion: The authors characterized a murine model of regional acid aspiration allowing long-term survival. Despite a partial recovery, at 2 weeks the Injury persisted, with evidence of fibrosis and lung compliance reduction. This long-term, low-mortality model seems suitable for assessment of the effects of different therapies on lung Injury and repair.

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