Lung matrix metalloproteinase activation following partial hepatic ischemia/reperfusion injury in rats

Giuseppina Palladini, Andrea Ferrigno, Vittoria Rizzo, Eleonora Tarantola, Vittorio Bertone, Isabel Freitas, Stefano Perlini, Plinio Richelmi, Mariapia Vairetti

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. After a 60 min reperfusion, liver, lung, kidney, and heart biopsies and blood samples were collected. Serum hepatic enzymes, creatinine, urea, Troponin I and TNF-alpha, and tissue matrix metalloproteinases (MMP-2, MMP-9), myeloperoxidase (MPO), malondialdehyde (MDA), and morphology were monitored. Results. Serum levels of hepatic enzymes and TNF-alpha were concomitantly increased during hepatic I/R. An increase in hepatic MMP-2 and MMP-9 activities was substantiated by tissue morphology alterations. Notably, acute hepatic I/R affect the lung inasmuch as MMP-9 activity and MPO levels were increased. No difference in MMPs and MPO was observed in kidney and heart. Conclusions. Although the underlying mechanism needs further investigation, this is the first study in which the MMP activation in a distant organ is reported; this event is probably TNF-alpha-mediated and the lung appears as the first remote organ to be involved in hepatic I/R injury.

Original languageEnglish
Article number867548
JournalTheScientificWorldJournal
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Reperfusion Injury
Matrix Metalloproteinases
Rats
Chemical activation
Lung
matrix
serum
Liver
enzyme
Peroxidase
urea
Tumor Necrosis Factor-alpha
Reperfusion
blood
Ischemia
Kidney
Tissue
Troponin I
organ
Biopsy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Lung matrix metalloproteinase activation following partial hepatic ischemia/reperfusion injury in rats. / Palladini, Giuseppina; Ferrigno, Andrea; Rizzo, Vittoria; Tarantola, Eleonora; Bertone, Vittorio; Freitas, Isabel; Perlini, Stefano; Richelmi, Plinio; Vairetti, Mariapia.

In: TheScientificWorldJournal, Vol. 2014, 867548, 2014.

Research output: Contribution to journalArticle

Palladini, Giuseppina ; Ferrigno, Andrea ; Rizzo, Vittoria ; Tarantola, Eleonora ; Bertone, Vittorio ; Freitas, Isabel ; Perlini, Stefano ; Richelmi, Plinio ; Vairetti, Mariapia. / Lung matrix metalloproteinase activation following partial hepatic ischemia/reperfusion injury in rats. In: TheScientificWorldJournal. 2014 ; Vol. 2014.
@article{48f0e5b1d4904b20994f6f78d45dd22e,
title = "Lung matrix metalloproteinase activation following partial hepatic ischemia/reperfusion injury in rats",
abstract = "Purpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. After a 60 min reperfusion, liver, lung, kidney, and heart biopsies and blood samples were collected. Serum hepatic enzymes, creatinine, urea, Troponin I and TNF-alpha, and tissue matrix metalloproteinases (MMP-2, MMP-9), myeloperoxidase (MPO), malondialdehyde (MDA), and morphology were monitored. Results. Serum levels of hepatic enzymes and TNF-alpha were concomitantly increased during hepatic I/R. An increase in hepatic MMP-2 and MMP-9 activities was substantiated by tissue morphology alterations. Notably, acute hepatic I/R affect the lung inasmuch as MMP-9 activity and MPO levels were increased. No difference in MMPs and MPO was observed in kidney and heart. Conclusions. Although the underlying mechanism needs further investigation, this is the first study in which the MMP activation in a distant organ is reported; this event is probably TNF-alpha-mediated and the lung appears as the first remote organ to be involved in hepatic I/R injury.",
author = "Giuseppina Palladini and Andrea Ferrigno and Vittoria Rizzo and Eleonora Tarantola and Vittorio Bertone and Isabel Freitas and Stefano Perlini and Plinio Richelmi and Mariapia Vairetti",
year = "2014",
doi = "10.1155/2014/867548",
language = "English",
volume = "2014",
journal = "The Scientific World Journal",
issn = "2356-6140",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Lung matrix metalloproteinase activation following partial hepatic ischemia/reperfusion injury in rats

AU - Palladini, Giuseppina

AU - Ferrigno, Andrea

AU - Rizzo, Vittoria

AU - Tarantola, Eleonora

AU - Bertone, Vittorio

AU - Freitas, Isabel

AU - Perlini, Stefano

AU - Richelmi, Plinio

AU - Vairetti, Mariapia

PY - 2014

Y1 - 2014

N2 - Purpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. After a 60 min reperfusion, liver, lung, kidney, and heart biopsies and blood samples were collected. Serum hepatic enzymes, creatinine, urea, Troponin I and TNF-alpha, and tissue matrix metalloproteinases (MMP-2, MMP-9), myeloperoxidase (MPO), malondialdehyde (MDA), and morphology were monitored. Results. Serum levels of hepatic enzymes and TNF-alpha were concomitantly increased during hepatic I/R. An increase in hepatic MMP-2 and MMP-9 activities was substantiated by tissue morphology alterations. Notably, acute hepatic I/R affect the lung inasmuch as MMP-9 activity and MPO levels were increased. No difference in MMPs and MPO was observed in kidney and heart. Conclusions. Although the underlying mechanism needs further investigation, this is the first study in which the MMP activation in a distant organ is reported; this event is probably TNF-alpha-mediated and the lung appears as the first remote organ to be involved in hepatic I/R injury.

AB - Purpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. After a 60 min reperfusion, liver, lung, kidney, and heart biopsies and blood samples were collected. Serum hepatic enzymes, creatinine, urea, Troponin I and TNF-alpha, and tissue matrix metalloproteinases (MMP-2, MMP-9), myeloperoxidase (MPO), malondialdehyde (MDA), and morphology were monitored. Results. Serum levels of hepatic enzymes and TNF-alpha were concomitantly increased during hepatic I/R. An increase in hepatic MMP-2 and MMP-9 activities was substantiated by tissue morphology alterations. Notably, acute hepatic I/R affect the lung inasmuch as MMP-9 activity and MPO levels were increased. No difference in MMPs and MPO was observed in kidney and heart. Conclusions. Although the underlying mechanism needs further investigation, this is the first study in which the MMP activation in a distant organ is reported; this event is probably TNF-alpha-mediated and the lung appears as the first remote organ to be involved in hepatic I/R injury.

UR - http://www.scopus.com/inward/record.url?scp=84893836291&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893836291&partnerID=8YFLogxK

U2 - 10.1155/2014/867548

DO - 10.1155/2014/867548

M3 - Article

C2 - 24592193

AN - SCOPUS:84893836291

VL - 2014

JO - The Scientific World Journal

JF - The Scientific World Journal

SN - 2356-6140

M1 - 867548

ER -