TY - JOUR
T1 - Lung myofibroblasts as targets of salmeterol and fluticasone propionate
T2 - inhibition of α-SMA and NF-κB
AU - Baouz, Soria
AU - Giron-Michel, Julien
AU - Azzarone, Bruno
AU - Giuliani, Massimo
AU - Cagnoni, Francesca
AU - Olsson, Susanna
AU - Testi, Renato
AU - Gabbiani, Giulio
AU - Canonica, G. Walter
PY - 2005/11
Y1 - 2005/11
N2 - Lung myofibroblasts play a major role in the pathophysiology of asthma, contributing not only to tissue remodelling but also to airway inflammation. Nevertheless, only recently, attention has been focused on these cells as potential targets for anti-allergic drugs. Herein, we analysed the pharmacological response of lung myofibroblasts to β2-agonists associated or not to inhaled corticosteroids, investigating their effects on (i) the constitutive and transforming growth factor-β (TGF-β)-induced expression of α-smooth muscle actin (α-SMA), the main functional marker of myofibroblastic differentiation and contractility; (ii) isometric contraction and (iii) tumour necrosis factor-α (TNF-α)-induced nuclear translocation of the pro-inflammatory transcription factor nuclear factor-κB (NF-κB). The β2-agonist salmeterol (SMl) has on human lung myofibroblasts new direct anti-contractile/anti-inflammatory effects that are amplified by the combined use of low concentrations of the glucocorticoid fluticasone propionate (FP). First, SMl and/or FP (10-12 M) inhibits the constitutive and TGF-β-induced expression of α-SMA. Second, the two drugs block the TNF-α-induced nuclear translocation of the pro-inflammatory transcription factor NF-κB. Finally, SMl decreases TNF-α-induced production of the inflammatory cytokine IL-6. The complementary anti-inflammatory/ anti-contractile effects displayed by SMl and FP on lung myofibroblasts in vitro may be related to the improvement in lung function and symptom control obtained in vivo by the early use of low doses of glucocorticoids in combination with long-acting β2-agonists.
AB - Lung myofibroblasts play a major role in the pathophysiology of asthma, contributing not only to tissue remodelling but also to airway inflammation. Nevertheless, only recently, attention has been focused on these cells as potential targets for anti-allergic drugs. Herein, we analysed the pharmacological response of lung myofibroblasts to β2-agonists associated or not to inhaled corticosteroids, investigating their effects on (i) the constitutive and transforming growth factor-β (TGF-β)-induced expression of α-smooth muscle actin (α-SMA), the main functional marker of myofibroblastic differentiation and contractility; (ii) isometric contraction and (iii) tumour necrosis factor-α (TNF-α)-induced nuclear translocation of the pro-inflammatory transcription factor nuclear factor-κB (NF-κB). The β2-agonist salmeterol (SMl) has on human lung myofibroblasts new direct anti-contractile/anti-inflammatory effects that are amplified by the combined use of low concentrations of the glucocorticoid fluticasone propionate (FP). First, SMl and/or FP (10-12 M) inhibits the constitutive and TGF-β-induced expression of α-SMA. Second, the two drugs block the TNF-α-induced nuclear translocation of the pro-inflammatory transcription factor NF-κB. Finally, SMl decreases TNF-α-induced production of the inflammatory cytokine IL-6. The complementary anti-inflammatory/ anti-contractile effects displayed by SMl and FP on lung myofibroblasts in vitro may be related to the improvement in lung function and symptom control obtained in vivo by the early use of low doses of glucocorticoids in combination with long-acting β2-agonists.
KW - β-agonists
KW - Airway inflammation
KW - Airway remodelling
KW - Asthma
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U2 - 10.1093/intimm/dxh325
DO - 10.1093/intimm/dxh325
M3 - Article
C2 - 16210331
AN - SCOPUS:27744441314
VL - 17
SP - 1473
EP - 1481
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 11
ER -