LY-6A/E gene is widely expressed among transformed nonhematopoietic cells. Autocrine modulation by interferon

P. L. Lollini, L. Landuzzi, G. Nlcoletti, C. De Giovanni, M. Glovarelli, E. Lalli, A. Facchini, P. Nanni

Research output: Contribution to journalArticlepeer-review


Expression of the Ly-6A/E gene by transformed cells was investigated in 14 cell cultures of C57BL/6 and BALB/c mouse origin derived from spontaneous or chemically-induced non-hematopoietic tumours and from cells transformed by SV40 virus. Histologic types included carcinomas, sarcomas and a melanoma. Thirteen out of 14 cell cultures expressed membrane Ly-6A/E antigens; only the B16-A melanoma was negative, but it expressed Ly-6A/E after incubation with IFN-γ. The effect of in vitro permanence was studied on early (20) passages of B16-A melanoma and MN/MCA1 fibrosarcoma. Late passage B16-A cells were Ly-6A/E-negative and refractory to induction of Ly-6A/E (but not of H-2 antigens) by IFN-α, IFN-β or IFN-γ; MN/MCA1 maintained a high expression of Ly-6A/E, but no increase was induced by IFNs. It was found that both early and late in vitro passages of MN/MCA1 actively produced IFN-α/β. The analysis of cells of fibroblastic origin revealed a significant correlation between IFN release in the culture medium and Ly-6A/E levels. Culture of fibrosarcoma cells in the presence of an anti-IFN-α/β serum reduced Ly-6A/E expression, thus indicating the existence of an autocrine loop.

Original languageEnglish
Pages (from-to)2245-2252
Number of pages8
JournalAnticancer Research
Issue number6 B
Publication statusPublished - 1992


  • Autocrine circuit
  • Interferon
  • Ly-6
  • Nonhematopoietic cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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