LY-6A/E gene is widely expressed among transformed nonhematopoietic cells. Autocrine modulation by interferon

P. L. Lollini, L. Landuzzi, G. Nlcoletti, C. De Giovanni, M. Glovarelli, E. Lalli, A. Facchini, P. Nanni

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Abstract

Expression of the Ly-6A/E gene by transformed cells was investigated in 14 cell cultures of C57BL/6 and BALB/c mouse origin derived from spontaneous or chemically-induced non-hematopoietic tumours and from cells transformed by SV40 virus. Histologic types included carcinomas, sarcomas and a melanoma. Thirteen out of 14 cell cultures expressed membrane Ly-6A/E antigens; only the B16-A melanoma was negative, but it expressed Ly-6A/E after incubation with IFN-γ. The effect of in vitro permanence was studied on early (20) passages of B16-A melanoma and MN/MCA1 fibrosarcoma. Late passage B16-A cells were Ly-6A/E-negative and refractory to induction of Ly-6A/E (but not of H-2 antigens) by IFN-α, IFN-β or IFN-γ; MN/MCA1 maintained a high expression of Ly-6A/E, but no increase was induced by IFNs. It was found that both early and late in vitro passages of MN/MCA1 actively produced IFN-α/β. The analysis of cells of fibroblastic origin revealed a significant correlation between IFN release in the culture medium and Ly-6A/E levels. Culture of fibrosarcoma cells in the presence of an anti-IFN-α/β serum reduced Ly-6A/E expression, thus indicating the existence of an autocrine loop.

Original languageEnglish
Pages (from-to)2245-2252
Number of pages8
JournalAnticancer Research
Volume12
Issue number6 B
Publication statusPublished - 1992

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Keywords

  • Autocrine circuit
  • Interferon
  • Ly-6
  • Nonhematopoietic cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lollini, P. L., Landuzzi, L., Nlcoletti, G., De Giovanni, C., Glovarelli, M., Lalli, E., Facchini, A., & Nanni, P. (1992). LY-6A/E gene is widely expressed among transformed nonhematopoietic cells. Autocrine modulation by interferon. Anticancer Research, 12(6 B), 2245-2252.