Lycopene, quercetin and tyrosol prevent macrophage activation induced by gliadin and IFN-γ

Daniela De Stefano, Maria Chiara Maiuri, Vittorio Simeon, Gianluca Grassia, Antonio Soscia, Maria Pia Cinelli, Rosa Carnuccio

Research output: Contribution to journalArticle

Abstract

Oxidative stress plays an important role in inflammatory process of celiac disease. We have studied the effect of the lycopene, quercetin and tyrosol natural antioxidants on the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expression in RAW 264.7 macrophages stimulated by gliadin in association with IFN-γ. The IFN-γ plus gliadin combination treatment was capable of enhancing iNOS and COX-2 gene expression and nuclear factor-κB (NF-κB), interferon regulatory factor-1 (IRF-1) and signal transducer and activator of transcription-1α (STAT-1α) activation induced by reactive oxygen species generation at 24 h. Lycopene, quercetin and tyrosol inhibited all these effects. The results here reported suggest that these compounds may represent non toxic agents for the control of pro-inflammatory genes involved in celiac disease.

Original languageEnglish
Pages (from-to)192-199
Number of pages8
JournalEuropean Journal of Pharmacology
Volume566
Issue number1-3
DOIs
Publication statusPublished - Jul 2 2007

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Keywords

  • Celiac disease
  • Gliadin
  • Interferon-gamma
  • RAW 264.7 macrophage
  • Transcription factors

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

De Stefano, D., Maiuri, M. C., Simeon, V., Grassia, G., Soscia, A., Cinelli, M. P., & Carnuccio, R. (2007). Lycopene, quercetin and tyrosol prevent macrophage activation induced by gliadin and IFN-γ. European Journal of Pharmacology, 566(1-3), 192-199. https://doi.org/10.1016/j.ejphar.2007.03.051