Studio sulla adesione di sottopopolazioni linfocitarie a cellule endoteliali cerebrali umane: Effetto di γ-IFN e desametazone

Translated title of the contribution: Lymhocyte subsets adhesion to human cerebral endothelial cells: Modulation by γ-IFN and dexamethazone

E. Corsini, M. Gelati, A. Dufour, S. Frigerio, E. Ciusani, G. Massa, C. Milanese, A. Nespolo, A. Salmaggi

Research output: Contribution to journalArticle

Abstract

Dexamethazone (Dx) is a synthetic steroid commonly used in treatment of multiple sclerosis (MS) relapses; it has a number of actions on immune cells, but it might also act on the endothelial cells preventing cellular adhesion at this level. We investigated the adhesion of CD4+ and CD8+ T-lymphocytes from healthy controls to in vitro cultured endothelial cells from human umbelical vein (HUVECs) and brain microvessels (HBECs). Endothelial cells were pretreated with Dx, γ-IFN or both prior to adhesion experiments. Adhering cells were evaluated by cytofluorimetric analysis. Endothelial cells supernates were assessed for β2-microglobulin and IL-1β levels. Both CD4+ and CD8+ cells adhesion increased after γ-IFN treatment of endothelial cells; co-stimulation with Dx did not inhibit this adhesion. In comparison with CD4+ and CD8+ percentages on PBMNCs, reduced percentages, expecially for CD4+ cells, were detected after adhesion (probably due to CD4 down regulation), β2-microglobulin levels in the supernates increased after γ- IFN stimulation and Dx was unable to counteract this action at 72 hours. On the other hand, Dx reduced IL-1β levels by HUVECs, also in costimulation with γ-IFN. No detectable levels of IL-1β were released by HBECs. Apart from the IL-1β release by HBECs, we did not detect major district specific differences between HUVECs and HBECs in the response to the applied stimulations.

Translated title of the contributionLymhocyte subsets adhesion to human cerebral endothelial cells: Modulation by γ-IFN and dexamethazone
Original languageItalian
Pages (from-to)435-441
Number of pages7
JournalRivista di Neurobiologia
Volume42
Issue number5-6
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Neuroscience(all)

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