Lymphocyte clones from old subjects: growing rate and functional activity

E. Mariani, P. Roda, A. R. Mariani, M. Vitale, A. Degrassi, S. Papa, F. A. Manzoli, A. Facchini

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Old subjects exhibit a decline in circulating T cells and an impaired proliferative response to mitogens, plus a relative increase in cells with NK phenotype not associated with a concomitant increase in their cytolitic activity. In the present study a limiting dilution assay was used to evaluate the phenotype, the functional activity and the proliferative capacity of clones obtained from peripheral blood lymphocytes of old and young subjects. CD5+ CD8+ clones from old people showed a significant impairment in their proliferative capacity and a decreased lytic activity against K562 and P815-IgG cell lines.

Original languageEnglish
Pages (from-to)103-105
Number of pages3
JournalCytotechnology
Volume1
Issue number1
DOIs
Publication statusPublished - Oct 1987

Fingerprint

T-cells
Lymphocytes
Mitogens
Dilution
Assays
Blood
Clone Cells
Immunoglobulin G
Cells
Phenotype
Natural Killer Cells
T-Lymphocytes
Cell Line

Keywords

  • aging
  • clone
  • lymphocyte
  • phenotype

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology

Cite this

Mariani, E., Roda, P., Mariani, A. R., Vitale, M., Degrassi, A., Papa, S., ... Facchini, A. (1987). Lymphocyte clones from old subjects: growing rate and functional activity. Cytotechnology, 1(1), 103-105. https://doi.org/10.1007/BF00351134

Lymphocyte clones from old subjects : growing rate and functional activity. / Mariani, E.; Roda, P.; Mariani, A. R.; Vitale, M.; Degrassi, A.; Papa, S.; Manzoli, F. A.; Facchini, A.

In: Cytotechnology, Vol. 1, No. 1, 10.1987, p. 103-105.

Research output: Contribution to journalArticle

Mariani, E, Roda, P, Mariani, AR, Vitale, M, Degrassi, A, Papa, S, Manzoli, FA & Facchini, A 1987, 'Lymphocyte clones from old subjects: growing rate and functional activity', Cytotechnology, vol. 1, no. 1, pp. 103-105. https://doi.org/10.1007/BF00351134
Mariani, E. ; Roda, P. ; Mariani, A. R. ; Vitale, M. ; Degrassi, A. ; Papa, S. ; Manzoli, F. A. ; Facchini, A. / Lymphocyte clones from old subjects : growing rate and functional activity. In: Cytotechnology. 1987 ; Vol. 1, No. 1. pp. 103-105.
@article{5c92ea458a30467d95f44f517b5df4fa,
title = "Lymphocyte clones from old subjects: growing rate and functional activity",
abstract = "Old subjects exhibit a decline in circulating T cells and an impaired proliferative response to mitogens, plus a relative increase in cells with NK phenotype not associated with a concomitant increase in their cytolitic activity. In the present study a limiting dilution assay was used to evaluate the phenotype, the functional activity and the proliferative capacity of clones obtained from peripheral blood lymphocytes of old and young subjects. CD5+ CD8+ clones from old people showed a significant impairment in their proliferative capacity and a decreased lytic activity against K562 and P815-IgG cell lines.",
keywords = "aging, clone, lymphocyte, phenotype",
author = "E. Mariani and P. Roda and Mariani, {A. R.} and M. Vitale and A. Degrassi and S. Papa and Manzoli, {F. A.} and A. Facchini",
year = "1987",
month = "10",
doi = "10.1007/BF00351134",
language = "English",
volume = "1",
pages = "103--105",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Lymphocyte clones from old subjects

T2 - growing rate and functional activity

AU - Mariani, E.

AU - Roda, P.

AU - Mariani, A. R.

AU - Vitale, M.

AU - Degrassi, A.

AU - Papa, S.

AU - Manzoli, F. A.

AU - Facchini, A.

PY - 1987/10

Y1 - 1987/10

N2 - Old subjects exhibit a decline in circulating T cells and an impaired proliferative response to mitogens, plus a relative increase in cells with NK phenotype not associated with a concomitant increase in their cytolitic activity. In the present study a limiting dilution assay was used to evaluate the phenotype, the functional activity and the proliferative capacity of clones obtained from peripheral blood lymphocytes of old and young subjects. CD5+ CD8+ clones from old people showed a significant impairment in their proliferative capacity and a decreased lytic activity against K562 and P815-IgG cell lines.

AB - Old subjects exhibit a decline in circulating T cells and an impaired proliferative response to mitogens, plus a relative increase in cells with NK phenotype not associated with a concomitant increase in their cytolitic activity. In the present study a limiting dilution assay was used to evaluate the phenotype, the functional activity and the proliferative capacity of clones obtained from peripheral blood lymphocytes of old and young subjects. CD5+ CD8+ clones from old people showed a significant impairment in their proliferative capacity and a decreased lytic activity against K562 and P815-IgG cell lines.

KW - aging

KW - clone

KW - lymphocyte

KW - phenotype

UR - http://www.scopus.com/inward/record.url?scp=0011764828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0011764828&partnerID=8YFLogxK

U2 - 10.1007/BF00351134

DO - 10.1007/BF00351134

M3 - Article

C2 - 22358452

AN - SCOPUS:0011764828

VL - 1

SP - 103

EP - 105

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 1

ER -