Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease

Dario Leosco, Francesca Fortunato, Giuseppe Rengo, Guido Iaccarino, Emma Sanzari, Luca Golino, Carmela Zincarelli, Vincenzo Canonico, Massimo Marchese, Walter J. Koch, Franco Rengo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Alterations in signal transduction pathway of G-protein-coupled receptors (GPCRs) have been found in the cerebrocortex and in the peripheral cultured tissues of patients with Alzheimer's disease (AD). The G-protein-coupled receptor kinase-2 (GRK2) plays an important role in regulating the GPCRs signaling: its increased expression is associated with receptor desensitization. The aim of this study was to explore GRK2 levels in peripheral lymphocytes of AD patients and to establish a correlation between lymphocyte protein concentrations and the degree of cognitive impairment. GRK2 mRNA and protein expression were evaluated in the lymphocytes of AD patients with mild or moderate/severe cognitive impairment and in age-matched healthy subjects. Both GRK2 mRNA and protein expression were higher in AD patients lymphocytes compared to controls. Furthermore, lymphocyte GRK2 levels were significantly correlated to the degree of cognitive decline. Our preliminary data suggest that GRK2 is involved in GPCRs coupling dysfunction observed in AD patients. Further studies are needed in order to verify whether the lymphocyte GRK2 might be utilized as a novel biomarker in AD diagnosis and clinical monitoring.

Original languageEnglish
Pages (from-to)279-282
Number of pages4
JournalNeuroscience Letters
Volume415
Issue number3
DOIs
Publication statusPublished - Mar 30 2007

Fingerprint

G-Protein-Coupled Receptor Kinase 2
Alzheimer Disease
Lymphocytes
G-Protein-Coupled Receptors
Messenger RNA
Proteins
Signal Transduction
Healthy Volunteers
Biomarkers

Keywords

  • Alzheimer's disease
  • Biological marker
  • Cognitive impairment
  • G-protein-coupled receptor kinases
  • G-protein-coupled receptors
  • Lymphocytes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease. / Leosco, Dario; Fortunato, Francesca; Rengo, Giuseppe; Iaccarino, Guido; Sanzari, Emma; Golino, Luca; Zincarelli, Carmela; Canonico, Vincenzo; Marchese, Massimo; Koch, Walter J.; Rengo, Franco.

In: Neuroscience Letters, Vol. 415, No. 3, 30.03.2007, p. 279-282.

Research output: Contribution to journalArticle

Leosco, D, Fortunato, F, Rengo, G, Iaccarino, G, Sanzari, E, Golino, L, Zincarelli, C, Canonico, V, Marchese, M, Koch, WJ & Rengo, F 2007, 'Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease', Neuroscience Letters, vol. 415, no. 3, pp. 279-282. https://doi.org/10.1016/j.neulet.2007.01.034
Leosco, Dario ; Fortunato, Francesca ; Rengo, Giuseppe ; Iaccarino, Guido ; Sanzari, Emma ; Golino, Luca ; Zincarelli, Carmela ; Canonico, Vincenzo ; Marchese, Massimo ; Koch, Walter J. ; Rengo, Franco. / Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease. In: Neuroscience Letters. 2007 ; Vol. 415, No. 3. pp. 279-282.
@article{95df8307998046c9b9c863295dbd059e,
title = "Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease",
abstract = "Alterations in signal transduction pathway of G-protein-coupled receptors (GPCRs) have been found in the cerebrocortex and in the peripheral cultured tissues of patients with Alzheimer's disease (AD). The G-protein-coupled receptor kinase-2 (GRK2) plays an important role in regulating the GPCRs signaling: its increased expression is associated with receptor desensitization. The aim of this study was to explore GRK2 levels in peripheral lymphocytes of AD patients and to establish a correlation between lymphocyte protein concentrations and the degree of cognitive impairment. GRK2 mRNA and protein expression were evaluated in the lymphocytes of AD patients with mild or moderate/severe cognitive impairment and in age-matched healthy subjects. Both GRK2 mRNA and protein expression were higher in AD patients lymphocytes compared to controls. Furthermore, lymphocyte GRK2 levels were significantly correlated to the degree of cognitive decline. Our preliminary data suggest that GRK2 is involved in GPCRs coupling dysfunction observed in AD patients. Further studies are needed in order to verify whether the lymphocyte GRK2 might be utilized as a novel biomarker in AD diagnosis and clinical monitoring.",
keywords = "Alzheimer's disease, Biological marker, Cognitive impairment, G-protein-coupled receptor kinases, G-protein-coupled receptors, Lymphocytes",
author = "Dario Leosco and Francesca Fortunato and Giuseppe Rengo and Guido Iaccarino and Emma Sanzari and Luca Golino and Carmela Zincarelli and Vincenzo Canonico and Massimo Marchese and Koch, {Walter J.} and Franco Rengo",
year = "2007",
month = "3",
day = "30",
doi = "10.1016/j.neulet.2007.01.034",
language = "English",
volume = "415",
pages = "279--282",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease

AU - Leosco, Dario

AU - Fortunato, Francesca

AU - Rengo, Giuseppe

AU - Iaccarino, Guido

AU - Sanzari, Emma

AU - Golino, Luca

AU - Zincarelli, Carmela

AU - Canonico, Vincenzo

AU - Marchese, Massimo

AU - Koch, Walter J.

AU - Rengo, Franco

PY - 2007/3/30

Y1 - 2007/3/30

N2 - Alterations in signal transduction pathway of G-protein-coupled receptors (GPCRs) have been found in the cerebrocortex and in the peripheral cultured tissues of patients with Alzheimer's disease (AD). The G-protein-coupled receptor kinase-2 (GRK2) plays an important role in regulating the GPCRs signaling: its increased expression is associated with receptor desensitization. The aim of this study was to explore GRK2 levels in peripheral lymphocytes of AD patients and to establish a correlation between lymphocyte protein concentrations and the degree of cognitive impairment. GRK2 mRNA and protein expression were evaluated in the lymphocytes of AD patients with mild or moderate/severe cognitive impairment and in age-matched healthy subjects. Both GRK2 mRNA and protein expression were higher in AD patients lymphocytes compared to controls. Furthermore, lymphocyte GRK2 levels were significantly correlated to the degree of cognitive decline. Our preliminary data suggest that GRK2 is involved in GPCRs coupling dysfunction observed in AD patients. Further studies are needed in order to verify whether the lymphocyte GRK2 might be utilized as a novel biomarker in AD diagnosis and clinical monitoring.

AB - Alterations in signal transduction pathway of G-protein-coupled receptors (GPCRs) have been found in the cerebrocortex and in the peripheral cultured tissues of patients with Alzheimer's disease (AD). The G-protein-coupled receptor kinase-2 (GRK2) plays an important role in regulating the GPCRs signaling: its increased expression is associated with receptor desensitization. The aim of this study was to explore GRK2 levels in peripheral lymphocytes of AD patients and to establish a correlation between lymphocyte protein concentrations and the degree of cognitive impairment. GRK2 mRNA and protein expression were evaluated in the lymphocytes of AD patients with mild or moderate/severe cognitive impairment and in age-matched healthy subjects. Both GRK2 mRNA and protein expression were higher in AD patients lymphocytes compared to controls. Furthermore, lymphocyte GRK2 levels were significantly correlated to the degree of cognitive decline. Our preliminary data suggest that GRK2 is involved in GPCRs coupling dysfunction observed in AD patients. Further studies are needed in order to verify whether the lymphocyte GRK2 might be utilized as a novel biomarker in AD diagnosis and clinical monitoring.

KW - Alzheimer's disease

KW - Biological marker

KW - Cognitive impairment

KW - G-protein-coupled receptor kinases

KW - G-protein-coupled receptors

KW - Lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=33947198179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947198179&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2007.01.034

DO - 10.1016/j.neulet.2007.01.034

M3 - Article

C2 - 17276003

AN - SCOPUS:33947198179

VL - 415

SP - 279

EP - 282

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -