Abstract
Lymphocyte cholinergic muscarinic receptors (MRs) and platelet monoamine oxidase-B (MAO-B) activity are considered surrogate markers of the same parameters in the central nervous system. Lymphocyte MR binding and platelet MAO-B activity were measured in a consistent number of healthy human adults and analysed according to gender and age. The mean value ± S.D. of MR binding neither differed between males (12.2 ± 10.0 fmol/106 cells, range: 0.5-37.9, n = 86) and females (10.7 ± 9.7 fmol/106 cells, range: 0.5-39.7, n = 69) nor among age groups. MAO-B activity was significantly higher in women (geometric mean: 11.3 nmol/mg protein/h, with 65% of values from 7.3 to 17.6; n = 43), than in men (7.7 nmol/mg protein/h, with 65% of values from 4.5 to 13; n = 95). Males aged 56-66 years displayed a higher, though not statistically significant, basal enzyme activity than younger subjects. Altogether these data indicate gender-related differences in MAO activity, but not in MR binding, and inter-individual differences in the basal values of both peripheral blood markers in healthy subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 715-720 |
| Number of pages | 6 |
| Journal | Environmental Toxicology and Pharmacology |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 2005 |
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Keywords
- Biomarkers
- Central nervous system
- Cholinergic system
- MAO-B
- Neurotoxicity
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
- Pharmacology
- Toxicology
Cite this
Lymphocyte muscarinic receptors and platelet monoamine oxidase-B as biomarkers of CNS function : Effects of age and gender in healthy humans. / Coccini, Teresa; Randine, Giovanna; Castoldi, Anna F.; Balloni, Laura; Baiardi, Paola; Manzo, Luigi.
In: Environmental Toxicology and Pharmacology, Vol. 19, No. 3, 05.2005, p. 715-720.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lymphocyte muscarinic receptors and platelet monoamine oxidase-B as biomarkers of CNS function
T2 - Effects of age and gender in healthy humans
AU - Coccini, Teresa
AU - Randine, Giovanna
AU - Castoldi, Anna F.
AU - Balloni, Laura
AU - Baiardi, Paola
AU - Manzo, Luigi
PY - 2005/5
Y1 - 2005/5
N2 - Lymphocyte cholinergic muscarinic receptors (MRs) and platelet monoamine oxidase-B (MAO-B) activity are considered surrogate markers of the same parameters in the central nervous system. Lymphocyte MR binding and platelet MAO-B activity were measured in a consistent number of healthy human adults and analysed according to gender and age. The mean value ± S.D. of MR binding neither differed between males (12.2 ± 10.0 fmol/106 cells, range: 0.5-37.9, n = 86) and females (10.7 ± 9.7 fmol/106 cells, range: 0.5-39.7, n = 69) nor among age groups. MAO-B activity was significantly higher in women (geometric mean: 11.3 nmol/mg protein/h, with 65% of values from 7.3 to 17.6; n = 43), than in men (7.7 nmol/mg protein/h, with 65% of values from 4.5 to 13; n = 95). Males aged 56-66 years displayed a higher, though not statistically significant, basal enzyme activity than younger subjects. Altogether these data indicate gender-related differences in MAO activity, but not in MR binding, and inter-individual differences in the basal values of both peripheral blood markers in healthy subjects.
AB - Lymphocyte cholinergic muscarinic receptors (MRs) and platelet monoamine oxidase-B (MAO-B) activity are considered surrogate markers of the same parameters in the central nervous system. Lymphocyte MR binding and platelet MAO-B activity were measured in a consistent number of healthy human adults and analysed according to gender and age. The mean value ± S.D. of MR binding neither differed between males (12.2 ± 10.0 fmol/106 cells, range: 0.5-37.9, n = 86) and females (10.7 ± 9.7 fmol/106 cells, range: 0.5-39.7, n = 69) nor among age groups. MAO-B activity was significantly higher in women (geometric mean: 11.3 nmol/mg protein/h, with 65% of values from 7.3 to 17.6; n = 43), than in men (7.7 nmol/mg protein/h, with 65% of values from 4.5 to 13; n = 95). Males aged 56-66 years displayed a higher, though not statistically significant, basal enzyme activity than younger subjects. Altogether these data indicate gender-related differences in MAO activity, but not in MR binding, and inter-individual differences in the basal values of both peripheral blood markers in healthy subjects.
KW - Biomarkers
KW - Central nervous system
KW - Cholinergic system
KW - MAO-B
KW - Neurotoxicity
UR - http://www.scopus.com/inward/record.url?scp=17044420896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17044420896&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2004.12.042
DO - 10.1016/j.etap.2004.12.042
M3 - Article
C2 - 21783547
AN - SCOPUS:17044420896
VL - 19
SP - 715
EP - 720
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
SN - 1382-6689
IS - 3
ER -