Lymphocyte muscarinic receptors and platelet monoamine oxidase-B as biomarkers of CNS function

Effects of age and gender in healthy humans

Teresa Coccini, Giovanna Randine, Anna F. Castoldi, Laura Balloni, Paola Baiardi, Luigi Manzo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Lymphocyte cholinergic muscarinic receptors (MRs) and platelet monoamine oxidase-B (MAO-B) activity are considered surrogate markers of the same parameters in the central nervous system. Lymphocyte MR binding and platelet MAO-B activity were measured in a consistent number of healthy human adults and analysed according to gender and age. The mean value ± S.D. of MR binding neither differed between males (12.2 ± 10.0 fmol/106 cells, range: 0.5-37.9, n = 86) and females (10.7 ± 9.7 fmol/106 cells, range: 0.5-39.7, n = 69) nor among age groups. MAO-B activity was significantly higher in women (geometric mean: 11.3 nmol/mg protein/h, with 65% of values from 7.3 to 17.6; n = 43), than in men (7.7 nmol/mg protein/h, with 65% of values from 4.5 to 13; n = 95). Males aged 56-66 years displayed a higher, though not statistically significant, basal enzyme activity than younger subjects. Altogether these data indicate gender-related differences in MAO activity, but not in MR binding, and inter-individual differences in the basal values of both peripheral blood markers in healthy subjects.

Original languageEnglish
Pages (from-to)715-720
Number of pages6
JournalEnvironmental Toxicology and Pharmacology
Volume19
Issue number3
DOIs
Publication statusPublished - May 2005

Fingerprint

Lymphocytes
Monoamine Oxidase
Biomarkers
Muscarinic Receptors
Platelets
Blood Platelets
Enzyme activity
Neurology
Cholinergic Receptors
Individuality
Cholinergic Agents
Healthy Volunteers
Proteins
Blood
Central Nervous System
Age Groups
Enzymes

Keywords

  • Biomarkers
  • Central nervous system
  • Cholinergic system
  • MAO-B
  • Neurotoxicity

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pharmacology
  • Toxicology

Cite this

Lymphocyte muscarinic receptors and platelet monoamine oxidase-B as biomarkers of CNS function : Effects of age and gender in healthy humans. / Coccini, Teresa; Randine, Giovanna; Castoldi, Anna F.; Balloni, Laura; Baiardi, Paola; Manzo, Luigi.

In: Environmental Toxicology and Pharmacology, Vol. 19, No. 3, 05.2005, p. 715-720.

Research output: Contribution to journalArticle

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