Lymphocyte subset patterns and cytokine production in Alzheimer's disease patients

Livianna Speciale, Elena Calabrese, Marina Saresella, Carmine Tinelli, Claudio Mariani, Lara Sanvito, Renato Longhi, Pasquale Ferrante

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

To investigate the signs of inflammatory processes in Alzheimer's disease (AD), we examined peripheral blood mononuclear cells (PBMC) from 51 AD patients (29 with mild and 22 with moderately severe dementia) and 51 age-matched healthy controls (HC), using flow cytometry to analyse the absolute number and the percentage of T, B and NK cells. We also studied the surface expression of CD25, CD28, CD57, CD71, CD45RA and CD45RO markers on cells CD4+ and CD8+. In 30 AD patients and 20 HC the production of IL-2, IFN-γ, IL-10 and TNF-α by PBMC after stimulation with [25-35], [1-40] and [1-16] beta-amyloid (βA) fragments was also evaluated. A significant decrease in circulating B and CD8+CD28- cells, as well as an increase in CD8+ cells expressing CD71+ and CD28+, was observed in AD patients. A significant decrease in IL-10 production was also found after stimulation of PMBC with βA [1-40]. The decreased IL-10 production was not related to disease severity. The observed imbalance of immune peripheral cell subpopulations and decreased IL-10 production point to a reduction of suppressor cell function in AD patients.

Original languageEnglish
Pages (from-to)1163-1169
Number of pages7
JournalNeurobiology of Aging
Volume28
Issue number8
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Lymphocyte Subsets
Alzheimer Disease
Interleukin-10
Cytokines
Blood Cells
Amyloid
Natural Killer Cells
Interleukin-2
Dementia
Flow Cytometry
B-Lymphocytes
T-Lymphocytes

Keywords

  • Alzheimer's disease
  • B cells
  • Beta-amyloid fragments
  • CD8+CD28+
  • CD8+CD28-
  • CD8+CD71+
  • IL-10

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Lymphocyte subset patterns and cytokine production in Alzheimer's disease patients. / Speciale, Livianna; Calabrese, Elena; Saresella, Marina; Tinelli, Carmine; Mariani, Claudio; Sanvito, Lara; Longhi, Renato; Ferrante, Pasquale.

In: Neurobiology of Aging, Vol. 28, No. 8, 08.2007, p. 1163-1169.

Research output: Contribution to journalArticle

Speciale, Livianna ; Calabrese, Elena ; Saresella, Marina ; Tinelli, Carmine ; Mariani, Claudio ; Sanvito, Lara ; Longhi, Renato ; Ferrante, Pasquale. / Lymphocyte subset patterns and cytokine production in Alzheimer's disease patients. In: Neurobiology of Aging. 2007 ; Vol. 28, No. 8. pp. 1163-1169.
@article{a6af766bc10e4d3aa6ac63f1f1d3e765,
title = "Lymphocyte subset patterns and cytokine production in Alzheimer's disease patients",
abstract = "To investigate the signs of inflammatory processes in Alzheimer's disease (AD), we examined peripheral blood mononuclear cells (PBMC) from 51 AD patients (29 with mild and 22 with moderately severe dementia) and 51 age-matched healthy controls (HC), using flow cytometry to analyse the absolute number and the percentage of T, B and NK cells. We also studied the surface expression of CD25, CD28, CD57, CD71, CD45RA and CD45RO markers on cells CD4+ and CD8+. In 30 AD patients and 20 HC the production of IL-2, IFN-γ, IL-10 and TNF-α by PBMC after stimulation with [25-35], [1-40] and [1-16] beta-amyloid (βA) fragments was also evaluated. A significant decrease in circulating B and CD8+CD28- cells, as well as an increase in CD8+ cells expressing CD71+ and CD28+, was observed in AD patients. A significant decrease in IL-10 production was also found after stimulation of PMBC with βA [1-40]. The decreased IL-10 production was not related to disease severity. The observed imbalance of immune peripheral cell subpopulations and decreased IL-10 production point to a reduction of suppressor cell function in AD patients.",
keywords = "Alzheimer's disease, B cells, Beta-amyloid fragments, CD8+CD28+, CD8+CD28-, CD8+CD71+, IL-10",
author = "Livianna Speciale and Elena Calabrese and Marina Saresella and Carmine Tinelli and Claudio Mariani and Lara Sanvito and Renato Longhi and Pasquale Ferrante",
year = "2007",
month = "8",
doi = "10.1016/j.neurobiolaging.2006.05.020",
language = "English",
volume = "28",
pages = "1163--1169",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Lymphocyte subset patterns and cytokine production in Alzheimer's disease patients

AU - Speciale, Livianna

AU - Calabrese, Elena

AU - Saresella, Marina

AU - Tinelli, Carmine

AU - Mariani, Claudio

AU - Sanvito, Lara

AU - Longhi, Renato

AU - Ferrante, Pasquale

PY - 2007/8

Y1 - 2007/8

N2 - To investigate the signs of inflammatory processes in Alzheimer's disease (AD), we examined peripheral blood mononuclear cells (PBMC) from 51 AD patients (29 with mild and 22 with moderately severe dementia) and 51 age-matched healthy controls (HC), using flow cytometry to analyse the absolute number and the percentage of T, B and NK cells. We also studied the surface expression of CD25, CD28, CD57, CD71, CD45RA and CD45RO markers on cells CD4+ and CD8+. In 30 AD patients and 20 HC the production of IL-2, IFN-γ, IL-10 and TNF-α by PBMC after stimulation with [25-35], [1-40] and [1-16] beta-amyloid (βA) fragments was also evaluated. A significant decrease in circulating B and CD8+CD28- cells, as well as an increase in CD8+ cells expressing CD71+ and CD28+, was observed in AD patients. A significant decrease in IL-10 production was also found after stimulation of PMBC with βA [1-40]. The decreased IL-10 production was not related to disease severity. The observed imbalance of immune peripheral cell subpopulations and decreased IL-10 production point to a reduction of suppressor cell function in AD patients.

AB - To investigate the signs of inflammatory processes in Alzheimer's disease (AD), we examined peripheral blood mononuclear cells (PBMC) from 51 AD patients (29 with mild and 22 with moderately severe dementia) and 51 age-matched healthy controls (HC), using flow cytometry to analyse the absolute number and the percentage of T, B and NK cells. We also studied the surface expression of CD25, CD28, CD57, CD71, CD45RA and CD45RO markers on cells CD4+ and CD8+. In 30 AD patients and 20 HC the production of IL-2, IFN-γ, IL-10 and TNF-α by PBMC after stimulation with [25-35], [1-40] and [1-16] beta-amyloid (βA) fragments was also evaluated. A significant decrease in circulating B and CD8+CD28- cells, as well as an increase in CD8+ cells expressing CD71+ and CD28+, was observed in AD patients. A significant decrease in IL-10 production was also found after stimulation of PMBC with βA [1-40]. The decreased IL-10 production was not related to disease severity. The observed imbalance of immune peripheral cell subpopulations and decreased IL-10 production point to a reduction of suppressor cell function in AD patients.

KW - Alzheimer's disease

KW - B cells

KW - Beta-amyloid fragments

KW - CD8+CD28+

KW - CD8+CD28-

KW - CD8+CD71+

KW - IL-10

UR - http://www.scopus.com/inward/record.url?scp=34249308473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249308473&partnerID=8YFLogxK

U2 - 10.1016/j.neurobiolaging.2006.05.020

DO - 10.1016/j.neurobiolaging.2006.05.020

M3 - Article

VL - 28

SP - 1163

EP - 1169

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

IS - 8

ER -