Lymphoma cell lines

In vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas)

H. G. Drexler, C. C. Uphoff, G. Gaidano, A. Carbone

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Primary effusion lymphoma (PEL; also known as body cavity-based lymphoma) is recognized as a new and unique lymphoma entity occurring predominantly, but not exclusively in human immunodeficiency virus (HIV)-seropositive patients with acquired immunodeficiency syndrome (AIDS). PEL grows exclusively in body cavities as serous lymphomatous effusion without evidence of mass disease or dissemination. Their most unique feature is infection with the newly discovered human herpesvirus-8 (HHV-8; also known as Kaposi's sarcoma-associated herpesvirus), often accompanied by co-infection with Epstein-Barr virus (EBV). A number of continuous lymphoma cell lines have been established from the malignant pleural effusion, ascitic fluid and peripheral blood of patients with AIDS- and non-AIDS-associated PEL. While all cell lines are HHV-8+, about half of them also contain EBV sequences. Stimulation of the cell lines causes switch from latent to lytic HHV-8 infection. The cells are generally negative for T and B cell immunomarkers (except for CD138 suggesting a pre- or terminal plasma cell stage) and positive for some activation and adhesion markers; they are genotypically B cells with their immunoglobulin genes rearranged. Complex, hyperdiploid karyotypes with multiple structural abnormalities are seen in the cell lines examined. No alterations of known proto-oncogenes are detected in PEL, with the exception of BCL-6 mutations occurring in a large percentage of cases. Heterotransplantation of the cell lines into immunodeficient mice leads to the development of lymphomatous effusion and marked angiogenesis. As HHV-8 contains DNA sequences of several protein homologues, the cell lines express various cytokines, cytokine receptors, chemokines, cell cycle and anti-apoptosis modulators which are upregulated upon stimulation. Indeed, some cell lines produce high levels of (human) interleukin-6 and interleukin-10. Taken together, these cell lines represent very important model systems for the elucidation of the pathobiology of PEL; furthermore, the cell lines are extremely useful scientific tools providing a resource to pursue studies of HHV-8-mediated pathogenic mechanisms.

Original languageEnglish
Pages (from-to)1507-1517
Number of pages11
JournalLeukemia
Volume12
Issue number10
Publication statusPublished - 1998

Fingerprint

Primary Effusion Lymphoma
Human Herpesvirus 8
Lymphoma
Cell Line
Human Herpesvirus 4
Acquired Immunodeficiency Syndrome
B-Lymphocytes
Malignant Pleural Effusion
Multiple Abnormalities
In Vitro Techniques
Immunoglobulin Genes
Cytokine Receptors
Polyploidy
Proto-Oncogenes
Ascitic Fluid
Infection
Plasma Cells
Coinfection
Karyotype
Chemokines

Keywords

  • Body cavity-based lymphoma
  • EBV
  • HHV-8
  • HIV
  • Primary effusion lymphomas
  • Viruses

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Drexler, H. G., Uphoff, C. C., Gaidano, G., & Carbone, A. (1998). Lymphoma cell lines: In vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas). Leukemia, 12(10), 1507-1517.

Lymphoma cell lines : In vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas). / Drexler, H. G.; Uphoff, C. C.; Gaidano, G.; Carbone, A.

In: Leukemia, Vol. 12, No. 10, 1998, p. 1507-1517.

Research output: Contribution to journalArticle

Drexler, HG, Uphoff, CC, Gaidano, G & Carbone, A 1998, 'Lymphoma cell lines: In vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas)', Leukemia, vol. 12, no. 10, pp. 1507-1517.
Drexler, H. G. ; Uphoff, C. C. ; Gaidano, G. ; Carbone, A. / Lymphoma cell lines : In vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas). In: Leukemia. 1998 ; Vol. 12, No. 10. pp. 1507-1517.
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