Lysis of autologous human melanoma cells by in vitro allosensitized peripheral blood lymphocytes

Giuseppe Fossati, Andrea Balsari, Donatella Taramelli, Maria Luisa Sensi, Giuseppe Pellegris, Maurizio Nava, Giorgio Parmiani

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Peripheral blood lymphocytes (PBL) of melanoma patients were sensitized in vitro with lymphocytes of a single donor or with a pool of lymphocytes of 5-20 different donors. After 6-7 days, the cytotoxic activity of the sensitized PBL was tested against cultured autologous tumor cells and lymphocytes in a 51Cr-release assay. Tumor lysis was observed in 13 of 16 cases in which patients' PBL (Pt-PBL) were stimulated by a pool of allogeneic lymphocytes and in five out of seven cases when single sensitization was performed. In no case was lysis of autologous normal lymphocytes or blasts seen. Cultivation of Pt-PBL with irradiated autologous tumor cells never led to the induction of lymphocytes cytotoxic to melanoma cells. Lysability by pool-activated autologous Pt-PBL of fresh cryopreserved tumor cells was compared to that of short-term cultured tumor cells, and no significant differences were observed. Cold-target inhibition experiments indicated that the cytotoxicity of Pt-PBL was tumor-restricted since only autologous melanoma cells but not lymphocytes were able to inhibit the reaction. These results indicate that activation of Pt-PBL is necessary in order to elicit or amplify their antitumor activity.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume14
Issue number2
DOIs
Publication statusPublished - Dec 1982

Fingerprint

Melanoma
Lymphocytes
Cultured Tumor Cells
Neoplasms
In Vitro Techniques
Tissue Donors

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

Cite this

Lysis of autologous human melanoma cells by in vitro allosensitized peripheral blood lymphocytes. / Fossati, Giuseppe; Balsari, Andrea; Taramelli, Donatella; Sensi, Maria Luisa; Pellegris, Giuseppe; Nava, Maurizio; Parmiani, Giorgio.

In: Cancer Immunology, Immunotherapy, Vol. 14, No. 2, 12.1982, p. 99-104.

Research output: Contribution to journalArticle

Fossati, Giuseppe ; Balsari, Andrea ; Taramelli, Donatella ; Sensi, Maria Luisa ; Pellegris, Giuseppe ; Nava, Maurizio ; Parmiani, Giorgio. / Lysis of autologous human melanoma cells by in vitro allosensitized peripheral blood lymphocytes. In: Cancer Immunology, Immunotherapy. 1982 ; Vol. 14, No. 2. pp. 99-104.
@article{49c87409ad164b9f9339a51390260e90,
title = "Lysis of autologous human melanoma cells by in vitro allosensitized peripheral blood lymphocytes",
abstract = "Peripheral blood lymphocytes (PBL) of melanoma patients were sensitized in vitro with lymphocytes of a single donor or with a pool of lymphocytes of 5-20 different donors. After 6-7 days, the cytotoxic activity of the sensitized PBL was tested against cultured autologous tumor cells and lymphocytes in a 51Cr-release assay. Tumor lysis was observed in 13 of 16 cases in which patients' PBL (Pt-PBL) were stimulated by a pool of allogeneic lymphocytes and in five out of seven cases when single sensitization was performed. In no case was lysis of autologous normal lymphocytes or blasts seen. Cultivation of Pt-PBL with irradiated autologous tumor cells never led to the induction of lymphocytes cytotoxic to melanoma cells. Lysability by pool-activated autologous Pt-PBL of fresh cryopreserved tumor cells was compared to that of short-term cultured tumor cells, and no significant differences were observed. Cold-target inhibition experiments indicated that the cytotoxicity of Pt-PBL was tumor-restricted since only autologous melanoma cells but not lymphocytes were able to inhibit the reaction. These results indicate that activation of Pt-PBL is necessary in order to elicit or amplify their antitumor activity.",
author = "Giuseppe Fossati and Andrea Balsari and Donatella Taramelli and Sensi, {Maria Luisa} and Giuseppe Pellegris and Maurizio Nava and Giorgio Parmiani",
year = "1982",
month = "12",
doi = "10.1007/BF00200176",
language = "English",
volume = "14",
pages = "99--104",
journal = "Cancer Immunology and Immunotherapy",
issn = "0340-7004",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "2",

}

TY - JOUR

T1 - Lysis of autologous human melanoma cells by in vitro allosensitized peripheral blood lymphocytes

AU - Fossati, Giuseppe

AU - Balsari, Andrea

AU - Taramelli, Donatella

AU - Sensi, Maria Luisa

AU - Pellegris, Giuseppe

AU - Nava, Maurizio

AU - Parmiani, Giorgio

PY - 1982/12

Y1 - 1982/12

N2 - Peripheral blood lymphocytes (PBL) of melanoma patients were sensitized in vitro with lymphocytes of a single donor or with a pool of lymphocytes of 5-20 different donors. After 6-7 days, the cytotoxic activity of the sensitized PBL was tested against cultured autologous tumor cells and lymphocytes in a 51Cr-release assay. Tumor lysis was observed in 13 of 16 cases in which patients' PBL (Pt-PBL) were stimulated by a pool of allogeneic lymphocytes and in five out of seven cases when single sensitization was performed. In no case was lysis of autologous normal lymphocytes or blasts seen. Cultivation of Pt-PBL with irradiated autologous tumor cells never led to the induction of lymphocytes cytotoxic to melanoma cells. Lysability by pool-activated autologous Pt-PBL of fresh cryopreserved tumor cells was compared to that of short-term cultured tumor cells, and no significant differences were observed. Cold-target inhibition experiments indicated that the cytotoxicity of Pt-PBL was tumor-restricted since only autologous melanoma cells but not lymphocytes were able to inhibit the reaction. These results indicate that activation of Pt-PBL is necessary in order to elicit or amplify their antitumor activity.

AB - Peripheral blood lymphocytes (PBL) of melanoma patients were sensitized in vitro with lymphocytes of a single donor or with a pool of lymphocytes of 5-20 different donors. After 6-7 days, the cytotoxic activity of the sensitized PBL was tested against cultured autologous tumor cells and lymphocytes in a 51Cr-release assay. Tumor lysis was observed in 13 of 16 cases in which patients' PBL (Pt-PBL) were stimulated by a pool of allogeneic lymphocytes and in five out of seven cases when single sensitization was performed. In no case was lysis of autologous normal lymphocytes or blasts seen. Cultivation of Pt-PBL with irradiated autologous tumor cells never led to the induction of lymphocytes cytotoxic to melanoma cells. Lysability by pool-activated autologous Pt-PBL of fresh cryopreserved tumor cells was compared to that of short-term cultured tumor cells, and no significant differences were observed. Cold-target inhibition experiments indicated that the cytotoxicity of Pt-PBL was tumor-restricted since only autologous melanoma cells but not lymphocytes were able to inhibit the reaction. These results indicate that activation of Pt-PBL is necessary in order to elicit or amplify their antitumor activity.

UR - http://www.scopus.com/inward/record.url?scp=0020467099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020467099&partnerID=8YFLogxK

U2 - 10.1007/BF00200176

DO - 10.1007/BF00200176

M3 - Article

VL - 14

SP - 99

EP - 104

JO - Cancer Immunology and Immunotherapy

JF - Cancer Immunology and Immunotherapy

SN - 0340-7004

IS - 2

ER -