Lysosomotropic agents induce morphological and functional changes in human muscle cells in vitro.

P. L. Baron, G. Meola, E. Scarpini, M. Moggio, I. Santilli, G. Scarlato

Research output: Contribution to journalArticle

Abstract

We studied the distribution of lysosomes in differentiating human muscle cells in culture treated with propranolol, leupeptin and chloroquine. Chloroquine treated cells showed a significant vacuolization and an increase of the lysosomal apparatus as assessed by cytochemical analysis of the lysosomal enzyme acid phosphatase and by acridine orange staining. At electron microscopic level, an increase of lysosome-like bodies and disorganization of the contractile apparatus were demonstrated in multinucleated myotubes. These alterations observed in cultured muscle cells suggest that lysosomotropic agents may be harmful.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalEuropean journal of basic and applied histochemistry
Volume35
Issue number4
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Medicine(all)

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