TY - JOUR
T1 - M. bovis infection in pigs
T2 - Improvement of the γ-IFN assay efficiency in this species using a maintenance medium
AU - Gerace, Elisabetta
AU - Mandanici, Francesca
AU - Pasquali, Paolo
AU - Falduto, Maria
AU - Vitale, Maria
AU - Di Marco Lo Presti, Vincenzo
AU - Amato, Benedetta
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The interferon-gamma (IFN-γ) test measures cell mediated immune response (CMI) during the early stages of tuberculosis infection. Although Bovine Tuberculosis (BT) spread in feral pigs is widely documented in literature, the effectiveness of IFN-γ in this species has been only recently reported. One of the major obstacle of this assay is that whole blood samples should be stimulated with purified protein derivative (PPD) cocktail within 8 h from the blood sampling. This study set up a defined broth culture in which lymphocytes, the cell population predominantly responsible for IFN-γ production, are maintained in a steady-state and their vitality is preserved. The IFN-γ production measured from the samples added with the maintenance medium and stored at 4 °C was similar to the enzyme-linked immunosorbent assay (ELISA) optical density values obtained from the same assay performed within 8 h from sampling.
AB - The interferon-gamma (IFN-γ) test measures cell mediated immune response (CMI) during the early stages of tuberculosis infection. Although Bovine Tuberculosis (BT) spread in feral pigs is widely documented in literature, the effectiveness of IFN-γ in this species has been only recently reported. One of the major obstacle of this assay is that whole blood samples should be stimulated with purified protein derivative (PPD) cocktail within 8 h from the blood sampling. This study set up a defined broth culture in which lymphocytes, the cell population predominantly responsible for IFN-γ production, are maintained in a steady-state and their vitality is preserved. The IFN-γ production measured from the samples added with the maintenance medium and stored at 4 °C was similar to the enzyme-linked immunosorbent assay (ELISA) optical density values obtained from the same assay performed within 8 h from sampling.
KW - Gamma interferon test (IFN-γ test)
KW - Mycobacterium bovis (M. bovis)
KW - Pigs
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U2 - 10.1016/j.tube.2017.12.001
DO - 10.1016/j.tube.2017.12.001
M3 - Article
C2 - 29523316
AN - SCOPUS:85037342921
VL - 108
SP - 151
EP - 154
JO - Tuberculosis
JF - Tuberculosis
SN - 1472-9792
ER -