M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2

Chiara Nicolò, Gabriele Di Sante, Annabella Procoli, Giuseppe Migliara, Alessia Piermattei, Mariagrazia Valentini, Giovanni Delogu, Achille Cittadini, Gabriela Constantin, Francesco Ria

Research output: Contribution to journalArticle

Abstract

DC deliver information regulating trafficking of effector T cells along T-cell priming. However, the role of pathogen-derived motives in the regulation of movement of T cells has not been studied. We hereinafter report that amount of M tuberculosis in the adjuvant modulates relocation of PLP139-151 specific T cells. In the presence of a low dose of M tuberculosis in the adjuvant, T cells (detected by CDR3 BV-BJ spectratyping, the so-called "immunoscope") mostly reach the spleen by day 28 after immunization ("late relocation") in the SJL strain, whereas T cells reach the spleen by d 14 with a high dose of M tuberculosis ("early relocation"). The C57Bl/6 background confers a dominant "early relocation" phenotype to F1 (SJL×C57Bl/6) mice, allowing early relocation of T cells in the presence of low dose M tuberculosis. A single non-synonymous polymorphism of TLR2 is responsible for "early/late" relocation phenotype. Egress of T lymphocytes is regulated by TLR2 expressed on T cells. Thus, pathogens engaging TLR2 on T cells regulate directly T-cell trafficking, and polymorphisms of TLR2 condition T-cell trafficking upon a limiting concentration of ligand.

Original languageEnglish
Article numbere55819
JournalPLoS One
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 11 2013

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2'. Together they form a unique fingerprint.

  • Cite this

    Nicolò, C., Di Sante, G., Procoli, A., Migliara, G., Piermattei, A., Valentini, M., Delogu, G., Cittadini, A., Constantin, G., & Ria, F. (2013). M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2. PLoS One, 8(2), [e55819]. https://doi.org/10.1371/journal.pone.0055819