M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2

Chiara Nicolò, Gabriele Di Sante, Annabella Procoli, Giuseppe Migliara, Alessia Piermattei, Mariagrazia Valentini, Giovanni Delogu, Achille Cittadini, Gabriela Constantin, Francesco Ria

Research output: Contribution to journalArticle

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Abstract

DC deliver information regulating trafficking of effector T cells along T-cell priming. However, the role of pathogen-derived motives in the regulation of movement of T cells has not been studied. We hereinafter report that amount of M tuberculosis in the adjuvant modulates relocation of PLP139-151 specific T cells. In the presence of a low dose of M tuberculosis in the adjuvant, T cells (detected by CDR3 BV-BJ spectratyping, the so-called "immunoscope") mostly reach the spleen by day 28 after immunization ("late relocation") in the SJL strain, whereas T cells reach the spleen by d 14 with a high dose of M tuberculosis ("early relocation"). The C57Bl/6 background confers a dominant "early relocation" phenotype to F1 (SJL×C57Bl/6) mice, allowing early relocation of T cells in the presence of low dose M tuberculosis. A single non-synonymous polymorphism of TLR2 is responsible for "early/late" relocation phenotype. Egress of T lymphocytes is regulated by TLR2 expressed on T cells. Thus, pathogens engaging TLR2 on T cells regulate directly T-cell trafficking, and polymorphisms of TLR2 condition T-cell trafficking upon a limiting concentration of ligand.

Original languageEnglish
Article numbere55819
JournalPLoS One
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 11 2013

Fingerprint

T-cells
tuberculosis
adjuvants
spleen
Tuberculosis
Spleen
T-lymphocytes
T-Lymphocytes
Relocation
Pathogens
Polymorphism
dosage
genetic polymorphism
Immunization
Phenotype
phenotype
pathogens
immunization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2. / Nicolò, Chiara; Di Sante, Gabriele; Procoli, Annabella; Migliara, Giuseppe; Piermattei, Alessia; Valentini, Mariagrazia; Delogu, Giovanni; Cittadini, Achille; Constantin, Gabriela; Ria, Francesco.

In: PLoS One, Vol. 8, No. 2, e55819, 11.02.2013.

Research output: Contribution to journalArticle

Nicolò, C, Di Sante, G, Procoli, A, Migliara, G, Piermattei, A, Valentini, M, Delogu, G, Cittadini, A, Constantin, G & Ria, F 2013, 'M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2', PLoS One, vol. 8, no. 2, e55819. https://doi.org/10.1371/journal.pone.0055819
Nicolò, Chiara ; Di Sante, Gabriele ; Procoli, Annabella ; Migliara, Giuseppe ; Piermattei, Alessia ; Valentini, Mariagrazia ; Delogu, Giovanni ; Cittadini, Achille ; Constantin, Gabriela ; Ria, Francesco. / M tuberculosis in the Adjuvant Modulates Time of Appearance of CNS-Specific Effector T Cells in the Spleen through a Polymorphic Site of TLR2. In: PLoS One. 2013 ; Vol. 8, No. 2.
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