mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP

P. Giacomini, R. Tecce, M. R. Nicotra, B. B. Cohen, M. C. Mazzilli, P. G. Natali

Research output: Contribution to journalArticle

Abstract

mAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα-mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specificities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain-associated forms, and (c) reacts with frozen, acetone-fixed, as well as conventional, formalin-fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue specimens.

Original languageEnglish
Pages (from-to)203-216
Number of pages14
JournalJournal of Immunogenetics
Volume16
Issue number3
Publication statusPublished - 1989

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Histocompatibility Antigens Class II
Tissue Distribution
Acetone
Paraffin
Haplotypes
Formaldehyde
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Giacomini, P., Tecce, R., Nicotra, M. R., Cohen, B. B., Mazzilli, M. C., & Natali, P. G. (1989). mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP. Journal of Immunogenetics, 16(3), 203-216.

mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP. / Giacomini, P.; Tecce, R.; Nicotra, M. R.; Cohen, B. B.; Mazzilli, M. C.; Natali, P. G.

In: Journal of Immunogenetics, Vol. 16, No. 3, 1989, p. 203-216.

Research output: Contribution to journalArticle

Giacomini, P, Tecce, R, Nicotra, MR, Cohen, BB, Mazzilli, MC & Natali, PG 1989, 'mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP', Journal of Immunogenetics, vol. 16, no. 3, pp. 203-216.
Giacomini, P. ; Tecce, R. ; Nicotra, M. R. ; Cohen, B. B. ; Mazzilli, M. C. ; Natali, P. G. / mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP. In: Journal of Immunogenetics. 1989 ; Vol. 16, No. 3. pp. 203-216.
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