Abstract
mAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα-mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specificities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain-associated forms, and (c) reacts with frozen, acetone-fixed, as well as conventional, formalin-fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 203-216 |
| Number of pages | 14 |
| Journal | Journal of Immunogenetics |
| Volume | 16 |
| Issue number | 3 |
| Publication status | Published - 1989 |
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ASJC Scopus subject areas
- Immunology
- Genetics
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mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP. / Giacomini, P.; Tecce, R.; Nicotra, M. R.; Cohen, B. B.; Mazzilli, M. C.; Natali, P. G.
In: Journal of Immunogenetics, Vol. 16, No. 3, 1989, p. 203-216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - mAb KUL/05 identifies a denaturation-resistant determinant shared by class II MHC products DR, DQ and DP
AU - Giacomini, P.
AU - Tecce, R.
AU - Nicotra, M. R.
AU - Cohen, B. B.
AU - Mazzilli, M. C.
AU - Natali, P. G.
PY - 1989
Y1 - 1989
N2 - mAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα-mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specificities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain-associated forms, and (c) reacts with frozen, acetone-fixed, as well as conventional, formalin-fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue specimens.
AB - mAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα-mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specificities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain-associated forms, and (c) reacts with frozen, acetone-fixed, as well as conventional, formalin-fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue specimens.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024849344&partnerID=8YFLogxK
M3 - Article
C2 - 2482314
AN - SCOPUS:0024849344
VL - 16
SP - 203
EP - 216
JO - Journal of Immunogenetics
JF - Journal of Immunogenetics
SN - 0305-1811
IS - 3
ER -