Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors

Andrea Ferrigno, Vittoria Rizzo, Eleonora Boncompagni, Alberto Bianchi, Enrico Gringeri, Daniele Neri, Plinio Richelmi, Isabel Freitas, Umberto Cillo, Mariapia Vairetti

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We previously reported that machine perfusion (MP) performed at 20 °C enhanced the preservation of steatotic rat livers. Here, we tested whether rat livers retrieved 30. min after cardiac arrest (NHBDs) were better protected by MP at 20 °C than with cold storage. We compared the recovery of livers from NHBDs with organs obtained from heart beating donors (HBDs) preserved by cold storage. MP technique: livers were perfused for 6. h with UW-G modified at 20 °C. Cold storage: livers were perfused in situ and preserved with UW solution at 4 °C for 6. h. Both MP and cold storage preserved livers were reperfused with Krebs-Heinselet buffer (2. h at 37 °C). AST and LDH release and mitochondrial glutamate dehydrogenase (GDH) levels were evaluated. Parameters assessed included: bile production and biliary enzymes; tissue ATP; reduced and oxidized glutathione (GSH/GSSG); protein-SH group concentration. Livers preserved by MP at 20 °C showed significantly lower hepatic damage at the end of reperfusion compared with cold storage. GDH release was significantly reduced and bile production, ATP levels, GSH/GSSG and protein-SH groups were higher in livers preserved by MP at 20 °C than with cold storage. The best preserved morphology and high glycogen content was obtained with livers submitted to MP at 20 °C. Liver recovery using MP at 20 °C was comparable to recovery with HBDs. MP at 20 °C improves cell survival and gives a better-quality of preservation for livers obtained from NHBDs and may provide a new method for the successful utilization of marginal livers.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalCryobiology
Volume62
Issue number2
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Liver
Perfusion
Cold storage
liver
cold storage
Glutathione Disulfide
Glutamate Dehydrogenase
glutamate dehydrogenase
Recovery
bile
Bile
Rats
Adenosine Triphosphate
heart
cardiac arrest
rats
Glycogen
Heart Arrest
Glutathione
glycogen

Keywords

  • Bile
  • Cold storage
  • Liver
  • Machine perfusion
  • Mitochondria
  • NHBD

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ferrigno, A., Rizzo, V., Boncompagni, E., Bianchi, A., Gringeri, E., Neri, D., ... Vairetti, M. (2011). Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors. Cryobiology, 62(2), 152-158. https://doi.org/10.1016/j.cryobiol.2011.02.004

Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors. / Ferrigno, Andrea; Rizzo, Vittoria; Boncompagni, Eleonora; Bianchi, Alberto; Gringeri, Enrico; Neri, Daniele; Richelmi, Plinio; Freitas, Isabel; Cillo, Umberto; Vairetti, Mariapia.

In: Cryobiology, Vol. 62, No. 2, 04.2011, p. 152-158.

Research output: Contribution to journalArticle

Ferrigno, A, Rizzo, V, Boncompagni, E, Bianchi, A, Gringeri, E, Neri, D, Richelmi, P, Freitas, I, Cillo, U & Vairetti, M 2011, 'Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors', Cryobiology, vol. 62, no. 2, pp. 152-158. https://doi.org/10.1016/j.cryobiol.2011.02.004
Ferrigno, Andrea ; Rizzo, Vittoria ; Boncompagni, Eleonora ; Bianchi, Alberto ; Gringeri, Enrico ; Neri, Daniele ; Richelmi, Plinio ; Freitas, Isabel ; Cillo, Umberto ; Vairetti, Mariapia. / Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors. In: Cryobiology. 2011 ; Vol. 62, No. 2. pp. 152-158.
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