Macrolide resistance mechanisms and expression of phenotypes among Streptococcus pneumoniae circulating in Italy

A. Marchese, E. Tonoli, E. A. Debbia, G. C. Schito

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In Italy, macrolide-resistant pneumococci have been isolated at a rate increasing from 6% in 1993 to 31.7% in 1998. A collection of 161 erythromycin-resistant Streptococcus pneumoniae recovered between 1993 and 1997 has now been phenotypically and genotypically characterized. Approximately 90% of these microorganisms possessed a constitutive MLS(B) mechanism of resistance. PCR detected ermB and mefE genes in strains showing MLS(B) and M phenotypes, respectively. Using pulsed-field gel electrophoresis of chromosomal DNA, one dominant restriction profile and its variations were detected in 51 S. pneumoniae isolates collected from different locations, indicating the circulation of a clone characterized by the possession of a great ability to spread.

Original languageEnglish
Pages (from-to)461-464
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume44
Issue number4
DOIs
Publication statusPublished - 1999

Fingerprint

Macrolides
Streptococcus pneumoniae
Italy
Phenotype
Pulsed Field Gel Electrophoresis
Erythromycin
Clone Cells
Polymerase Chain Reaction
DNA
Genes

ASJC Scopus subject areas

  • Microbiology
  • Pharmacology

Cite this

Macrolide resistance mechanisms and expression of phenotypes among Streptococcus pneumoniae circulating in Italy. / Marchese, A.; Tonoli, E.; Debbia, E. A.; Schito, G. C.

In: Journal of Antimicrobial Chemotherapy, Vol. 44, No. 4, 1999, p. 461-464.

Research output: Contribution to journalArticle

Marchese, A. ; Tonoli, E. ; Debbia, E. A. ; Schito, G. C. / Macrolide resistance mechanisms and expression of phenotypes among Streptococcus pneumoniae circulating in Italy. In: Journal of Antimicrobial Chemotherapy. 1999 ; Vol. 44, No. 4. pp. 461-464.
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