Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines

Peter J. Murray; Judith E. Allen; Subhra K. Biswas; Edward A. Fisher; Derek W. Gilroy; Sergij Goerdt; Siamon Gordon; John A. Hamilton; Lionel B. Ivashkiv; Toby Lawrence; Massimo Locati; Alberto Mantovani; Fernando O. Martinez; Jean Louis Mege; David M. Mosser; Gioacchino Natoli; Jeroen P. Saeij; Joachim L. Schultze; KariAnn Shirey; Antonio Sica; Jill Suttles; Irina Udalova; Jo A. vanGinderachter; Stefanie N. Vogel; Thomas A. Wynn

doi:10.1016/j.immuni.2014.06.008

Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines

Peter J. Murray, Judith E. Allen, Subhra K. Biswas, Edward A. Fisher, Derek W. Gilroy, Sergij Goerdt, Siamon Gordon, John A. Hamilton, Lionel B. Ivashkiv, Toby Lawrence, Massimo Locati, Alberto Mantovani, Fernando O. Martinez, Jean Louis Mege, David M. Mosser, Gioacchino Natoli, Jeroen P. Saeij, Joachim L. Schultze, KariAnn Shirey, Antonio SicaJill Suttles, Irina Udalova, Jo A. vanGinderachter, Stefanie N. Vogel, Thomas A. Wynn

Research output: Contribution to journal › Article › peer-review

Abstract

Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments invitro and invivo impedes progress in multiple ways, including the fact that many researchers still consider there to be only two types of activated macrophages, often termed M1 and M2. Here, we describe a set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation-with the goal of unifying experimental standards for diverse experimental scenarios. Collectively, we propose a common framework for macrophage-activation nomenclature. The description of macrophage activation status is contentious and confusing. Murray etal. propose a framework for macrophage-activation nomenclature.

Original language	English
Pages (from-to)	14-20
Number of pages	7
Journal	Immunity
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.immuni.2014.06.008
Publication status	Published - Jul 17 2014

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases
Immunology

Access to Document

10.1016/j.immuni.2014.06.008

Cite this

Murray, P. J., Allen, J. E., Biswas, S. K., Fisher, E. A., Gilroy, D. W., Goerdt, S., Gordon, S., Hamilton, J. A., Ivashkiv, L. B., Lawrence, T., Locati, M., Mantovani, A., Martinez, F. O., Mege, J. L., Mosser, D. M., Natoli, G., Saeij, J. P., Schultze, J. L., Shirey, K., ... Wynn, T. A. (2014). Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines. Immunity, 41(1), 14-20. https://doi.org/10.1016/j.immuni.2014.06.008

Macrophage Activation and Polarization : Nomenclature and Experimental Guidelines. / Murray, Peter J.; Allen, Judith E.; Biswas, Subhra K.; Fisher, Edward A.; Gilroy, Derek W.; Goerdt, Sergij; Gordon, Siamon; Hamilton, John A.; Ivashkiv, Lionel B.; Lawrence, Toby; Locati, Massimo ; Mantovani, Alberto; Martinez, Fernando O.; Mege, Jean Louis; Mosser, David M.; Natoli, Gioacchino; Saeij, Jeroen P.; Schultze, Joachim L.; Shirey, KariAnn; Sica, Antonio; Suttles, Jill; Udalova, Irina; vanGinderachter, Jo A.; Vogel, Stefanie N.; Wynn, Thomas A.

In: Immunity, Vol. 41, No. 1, 17.07.2014, p. 14-20.

Research output: Contribution to journal › Article › peer-review

Murray, PJ, Allen, JE, Biswas, SK, Fisher, EA, Gilroy, DW, Goerdt, S, Gordon, S, Hamilton, JA, Ivashkiv, LB, Lawrence, T, Locati, M , Mantovani, A, Martinez, FO, Mege, JL, Mosser, DM, Natoli, G, Saeij, JP, Schultze, JL, Shirey, K, Sica, A, Suttles, J, Udalova, I, vanGinderachter, JA, Vogel, SN & Wynn, TA 2014, 'Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines', Immunity, vol. 41, no. 1, pp. 14-20. https://doi.org/10.1016/j.immuni.2014.06.008

Murray, Peter J. ; Allen, Judith E. ; Biswas, Subhra K. ; Fisher, Edward A. ; Gilroy, Derek W. ; Goerdt, Sergij ; Gordon, Siamon ; Hamilton, John A. ; Ivashkiv, Lionel B. ; Lawrence, Toby ; Locati, Massimo ; Mantovani, Alberto ; Martinez, Fernando O. ; Mege, Jean Louis ; Mosser, David M. ; Natoli, Gioacchino ; Saeij, Jeroen P. ; Schultze, Joachim L. ; Shirey, KariAnn ; Sica, Antonio ; Suttles, Jill ; Udalova, Irina ; vanGinderachter, Jo A. ; Vogel, Stefanie N. ; Wynn, Thomas A. / Macrophage Activation and Polarization : Nomenclature and Experimental Guidelines. In: Immunity. 2014 ; Vol. 41, No. 1. pp. 14-20.

@article{a9449ca2e0374a0f9e328cf15eb28e20,

title = "Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines",

abstract = "Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments invitro and invivo impedes progress in multiple ways, including the fact that many researchers still consider there to be only two types of activated macrophages, often termed M1 and M2. Here, we describe a set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation-with the goal of unifying experimental standards for diverse experimental scenarios. Collectively, we propose a common framework for macrophage-activation nomenclature. The description of macrophage activation status is contentious and confusing. Murray etal. propose a framework for macrophage-activation nomenclature.",

author = "Murray, {Peter J.} and Allen, {Judith E.} and Biswas, {Subhra K.} and Fisher, {Edward A.} and Gilroy, {Derek W.} and Sergij Goerdt and Siamon Gordon and Hamilton, {John A.} and Ivashkiv, {Lionel B.} and Toby Lawrence and Massimo Locati and Alberto Mantovani and Martinez, {Fernando O.} and Mege, {Jean Louis} and Mosser, {David M.} and Gioacchino Natoli and Saeij, {Jeroen P.} and Schultze, {Joachim L.} and KariAnn Shirey and Antonio Sica and Jill Suttles and Irina Udalova and vanGinderachter, {Jo A.} and Vogel, {Stefanie N.} and Wynn, {Thomas A.}",

year = "2014",

month = jul,

day = "17",

doi = "10.1016/j.immuni.2014.06.008",

language = "English",

volume = "41",

pages = "14--20",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Macrophage Activation and Polarization

T2 - Nomenclature and Experimental Guidelines

AU - Murray, Peter J.

AU - Allen, Judith E.

AU - Biswas, Subhra K.

AU - Fisher, Edward A.

AU - Gilroy, Derek W.

AU - Goerdt, Sergij

AU - Gordon, Siamon

AU - Hamilton, John A.

AU - Ivashkiv, Lionel B.

AU - Lawrence, Toby

AU - Locati, Massimo

AU - Mantovani, Alberto

AU - Martinez, Fernando O.

AU - Mege, Jean Louis

AU - Mosser, David M.

AU - Natoli, Gioacchino

AU - Saeij, Jeroen P.

AU - Schultze, Joachim L.

AU - Shirey, KariAnn

AU - Sica, Antonio

AU - Suttles, Jill

AU - Udalova, Irina

AU - vanGinderachter, Jo A.

AU - Vogel, Stefanie N.

AU - Wynn, Thomas A.

PY - 2014/7/17

Y1 - 2014/7/17

N2 - Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments invitro and invivo impedes progress in multiple ways, including the fact that many researchers still consider there to be only two types of activated macrophages, often termed M1 and M2. Here, we describe a set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation-with the goal of unifying experimental standards for diverse experimental scenarios. Collectively, we propose a common framework for macrophage-activation nomenclature. The description of macrophage activation status is contentious and confusing. Murray etal. propose a framework for macrophage-activation nomenclature.

AB - Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments invitro and invivo impedes progress in multiple ways, including the fact that many researchers still consider there to be only two types of activated macrophages, often termed M1 and M2. Here, we describe a set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation-with the goal of unifying experimental standards for diverse experimental scenarios. Collectively, we propose a common framework for macrophage-activation nomenclature. The description of macrophage activation status is contentious and confusing. Murray etal. propose a framework for macrophage-activation nomenclature.

UR - http://www.scopus.com/inward/record.url?scp=84904394690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904394690&partnerID=8YFLogxK

U2 - 10.1016/j.immuni.2014.06.008

DO - 10.1016/j.immuni.2014.06.008

M3 - Article

C2 - 25035950

AN - SCOPUS:84904394690

VL - 41

SP - 14

EP - 20

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 1

ER -

Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this