Macrophage infiltration and death in the nerve during the early phases of experimental diabetic neuropathy: A process concomitant with endoneurial induction of IL-1β and p75NTR

Giancarlo Conti, Elio Scarpini, Pierluigi Baron, Simona Livraghi, Marco Tiriticco, Roberto Bianchi, Christian Vedeler, Guglielmo Scarlato

Research output: Contribution to journalArticlepeer-review

Abstract

This study describes the infiltration and death of monocyte/macrophages and concomitant endoneurial expression of the pro-inflammatory cytokine interleukin-1beta (IL-1β) and neurotrophin receptor p75 (p75NTR) in the sciatic nerve at the early phases of experimental diabetic neuropathy induced in Lewis rats by streptozotocin (STZ) intraperitoneal injection. Immunocytochemistry and single nerve fiber immunostaining showed the presence of macrophages in diabetic nerves by weeks 2 and 3 after STZ administration, and the 15% of these cells were TUNEL positive. IL-1β was evident in scattered macrophages, and along few isolated nerve fibers until week 5, when it became undetectable, in concomitance with complete endoneurial clearance of macrophages. p75NTR showed an up-regulation in the sciatic nerve of diabetic rats that began by week 3 after STZ administration, reached its peak by week 5, and returned then to a barely detectable level by week 6. These findings seem to indicate that macrophages and IL-1β may be involved in the pathogenesis of diabetic neuropathy, participating not only to nerve damage but also to the promotion of an attempt of regeneration via p75NTR induction.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalJournal of the Neurological Sciences
Volume195
Issue number1
Publication statusPublished - Mar 15 2002

Keywords

  • Apoptosis
  • Experimental diabetic neuropathy
  • IL-1β
  • Macrophage
  • p75NTR
  • Peripheral nerve

ASJC Scopus subject areas

  • Ageing
  • Clinical Neurology
  • Surgery
  • Developmental Neuroscience
  • Neurology
  • Neuroscience(all)

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