TY - JOUR
T1 - Macrophage plasticity in experimental atherosclerosis
AU - Khallou-Laschet, Jamila
AU - Varthaman, Aditi
AU - Fornasa, Giulia
AU - Compain, Caroline
AU - Gaston, Anh Thu
AU - Clement, Marc
AU - Dussiot, Michaël
AU - Levillain, Olivier
AU - Graff-Dubois, Stéphanie
AU - Nicoletti, Antonino
AU - Caligiuri, Giuseppina
PY - 2010/1/25
Y1 - 2010/1/25
N2 - As in human disease, macrophages (M∅) are central players in the development and progression of experimental atherosclerosis. In this study we have evaluated the phenotype of M∅ associated with progression of atherosclerosis in the apolipoprotein E (ApoE) knockout (KO) mouse model. We found that bone marrow-derived M∅ submitted to M1 and M2 polarization specifically expressed arginase (Arg) II and Arg I, respectively. This distinct arginase expression was used to evaluate the frequency and distribution of M1 and M2 M∅ in cross-sections of atherosclerotic plaques of ApoE KO mice. Early lesions were infiltrated by Arg I+ (M2) M∅. This type of M∅ favored the proliferation of smooth muscle cells, in vitro. Arg II+ (M1) M∅ appeared and prevailed in lesions of aged ApoE KO mice and lesion progression was correlated with the dominance of M1 over the M2 M∅ phenotype. In order to address whether the M2-.M1 switch could be due to a phenotypic switch of the infiltrated cells, we performed in vitro repolarization experiments. We found that fully polarized M∅ retained their plasticity since they could revert their phenotype. The analysis of the distribution of Arg Iand Arg II-expressing M∅ also argued against a recent recruitment of M1 M∅ in the lesion. The combined data therefore suggest that the M2-.M1 switch observed in vivo is due to a conversion of cells already present in the lesion. Our study suggests that interventional tools able to revert the M∅ infiltrate towards the M2 phenotype may exert an atheroprotective action. Copyright:
AB - As in human disease, macrophages (M∅) are central players in the development and progression of experimental atherosclerosis. In this study we have evaluated the phenotype of M∅ associated with progression of atherosclerosis in the apolipoprotein E (ApoE) knockout (KO) mouse model. We found that bone marrow-derived M∅ submitted to M1 and M2 polarization specifically expressed arginase (Arg) II and Arg I, respectively. This distinct arginase expression was used to evaluate the frequency and distribution of M1 and M2 M∅ in cross-sections of atherosclerotic plaques of ApoE KO mice. Early lesions were infiltrated by Arg I+ (M2) M∅. This type of M∅ favored the proliferation of smooth muscle cells, in vitro. Arg II+ (M1) M∅ appeared and prevailed in lesions of aged ApoE KO mice and lesion progression was correlated with the dominance of M1 over the M2 M∅ phenotype. In order to address whether the M2-.M1 switch could be due to a phenotypic switch of the infiltrated cells, we performed in vitro repolarization experiments. We found that fully polarized M∅ retained their plasticity since they could revert their phenotype. The analysis of the distribution of Arg Iand Arg II-expressing M∅ also argued against a recent recruitment of M1 M∅ in the lesion. The combined data therefore suggest that the M2-.M1 switch observed in vivo is due to a conversion of cells already present in the lesion. Our study suggests that interventional tools able to revert the M∅ infiltrate towards the M2 phenotype may exert an atheroprotective action. Copyright:
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U2 - 10.1371/journal.pone.0008852
DO - 10.1371/journal.pone.0008852
M3 - Article
C2 - 20111605
AN - SCOPUS:77952532171
VL - 5
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 1
M1 - e8852
ER -