Macrophages and fibrosis in adipose tissue are linked to liver damage and metabolic risk in obese children

Ryan W. Walker, Hooman Allayee, Alessandro Inserra, Rodolfo Fruhwirth, Anna Alisi, Rita Devito, Magalie E. Carey, Frank Sinatra, Michael I. Goran, Valerio Nobili

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective Obesity in childhood is associated with an inflammatory state in adipose tissue and liver, which elevates risk for diabetes and liver disease. No prior study has examined associations between pathologies occurring in adipose tissue and liver to identify elements of tissue damage associated with type 2 diabetes risk. This study sought to determine whether inflammation and fibrosis in abdominal subcutaneous adipose tissue (SAT) in obese/overweight children (BMI-z 2.3±0.76) was related to the extent of observed liver disease or type 2 diabetes risk. Methods Biopsy samples of abdominal (SAT) and liver were simultaneously collected from 33 Italian children (mean BMI 28.1±5.1 kg/m2 and mean age 11.6±2.2 years) with confirmed NAFLD. Histology and immunohistochemistry were conducted on biopsies to assess inflammation and fibrosis in adipose tissue and fibrosis and inflammation in liver. Results Presence vs. absence of crown-like structures (CLS) in SAT was significantly related to liver fibrosis scores (1.7±0.7 vs. 1.2±0.7, P=0.04) independent of BMI. SAT fibrosis was significantly correlated with a lower disposition index (r=-0.48, P=0.006). No other adipose measures were associated with liver disease parameters. Conclusion Markers of subcutaneous white adipose tissue inflammation are associated with greater extent of liver fibrosis independent of obesity and SAT fibrosis may contribute to diabetes risk through reduced insulin secretion.

Original languageEnglish
Pages (from-to)1512-1519
Number of pages8
JournalObesity
Volume22
Issue number6
DOIs
Publication statusPublished - 2014

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Subcutaneous Fat
Adipose Tissue
Fibrosis
Macrophages
Abdominal Subcutaneous Fat
Liver
Inflammation
Liver Diseases
Liver Cirrhosis
Type 2 Diabetes Mellitus
Biopsy
White Adipose Tissue
Pediatric Obesity
Crowns
Histology
Obesity
Immunohistochemistry
Insulin
Pathology

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics
  • Endocrinology
  • Medicine(all)

Cite this

Macrophages and fibrosis in adipose tissue are linked to liver damage and metabolic risk in obese children. / Walker, Ryan W.; Allayee, Hooman; Inserra, Alessandro; Fruhwirth, Rodolfo; Alisi, Anna; Devito, Rita; Carey, Magalie E.; Sinatra, Frank; Goran, Michael I.; Nobili, Valerio.

In: Obesity, Vol. 22, No. 6, 2014, p. 1512-1519.

Research output: Contribution to journalArticle

Walker, Ryan W. ; Allayee, Hooman ; Inserra, Alessandro ; Fruhwirth, Rodolfo ; Alisi, Anna ; Devito, Rita ; Carey, Magalie E. ; Sinatra, Frank ; Goran, Michael I. ; Nobili, Valerio. / Macrophages and fibrosis in adipose tissue are linked to liver damage and metabolic risk in obese children. In: Obesity. 2014 ; Vol. 22, No. 6. pp. 1512-1519.
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