Madin Darby Canine Kidney: A new cell line for Pneumocystis capinii in vitro culture

C. Atzori; A. Bruno; C. Agostoni; S. Novati; S. Gatti; M. Scaglia

Madin Darby Canine Kidney: A new cell line for Pneumocystis capinii in vitro culture

C. Atzori, A. Bruno, C. Agostoni, S. Novati, S. Gatti, M. Scaglia

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Pneumocystis carinii pneumonia (PCP) is a highly frequent cause of morbidity and mortality in immunocompromised subjects, particularly in HIV-infected ones. The biology of P. carinii is poorly understood because of the lack of reiable synthetic media or adequate cell lines to grow this opportunistic pathogen in continuous culture. We reported the suitability of the MDCK (Madin Darby Canine Kidney, ATTC CCL 34) cell line to support the temporary microorganism's growth in vitro and the experimental pharmacological trials, in comparison with the HEL 299 cell line, used as reference standard.

Original language	English
Pages (from-to)	183-185
Number of pages	3
Journal	Parasite (Paris, France)
Volume	3
Issue number	2
Publication status	Published - 1996

Keywords

In vitro culture
MDCK cell line
Pneumocystis carinii

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Parasitology

Access to Document

Fingerprint Dive into the research topics of 'Madin Darby Canine Kidney: A new cell line for Pneumocystis capinii in vitro culture'. Together they form a unique fingerprint.

Cite this

@article{4554e0cd1aa04e10bbd48567bf894a11,

title = "Madin Darby Canine Kidney: A new cell line for Pneumocystis capinii in vitro culture",

abstract = "Pneumocystis carinii pneumonia (PCP) is a highly frequent cause of morbidity and mortality in immunocompromised subjects, particularly in HIV-infected ones. The biology of P. carinii is poorly understood because of the lack of reiable synthetic media or adequate cell lines to grow this opportunistic pathogen in continuous culture. We reported the suitability of the MDCK (Madin Darby Canine Kidney, ATTC CCL 34) cell line to support the temporary microorganism's growth in vitro and the experimental pharmacological trials, in comparison with the HEL 299 cell line, used as reference standard.",

keywords = "In vitro culture, MDCK cell line, Pneumocystis carinii",

author = "C. Atzori and A. Bruno and C. Agostoni and S. Novati and S. Gatti and M. Scaglia",

year = "1996",

language = "English",

volume = "3",

pages = "183--185",

journal = "Parasite",

issn = "1252-607X",

publisher = "EDP Sciences",

number = "2",

}

TY - JOUR

T1 - Madin Darby Canine Kidney

T2 - A new cell line for Pneumocystis capinii in vitro culture

AU - Atzori, C.

AU - Bruno, A.

AU - Agostoni, C.

AU - Novati, S.

AU - Gatti, S.

AU - Scaglia, M.

PY - 1996

Y1 - 1996

N2 - Pneumocystis carinii pneumonia (PCP) is a highly frequent cause of morbidity and mortality in immunocompromised subjects, particularly in HIV-infected ones. The biology of P. carinii is poorly understood because of the lack of reiable synthetic media or adequate cell lines to grow this opportunistic pathogen in continuous culture. We reported the suitability of the MDCK (Madin Darby Canine Kidney, ATTC CCL 34) cell line to support the temporary microorganism's growth in vitro and the experimental pharmacological trials, in comparison with the HEL 299 cell line, used as reference standard.

AB - Pneumocystis carinii pneumonia (PCP) is a highly frequent cause of morbidity and mortality in immunocompromised subjects, particularly in HIV-infected ones. The biology of P. carinii is poorly understood because of the lack of reiable synthetic media or adequate cell lines to grow this opportunistic pathogen in continuous culture. We reported the suitability of the MDCK (Madin Darby Canine Kidney, ATTC CCL 34) cell line to support the temporary microorganism's growth in vitro and the experimental pharmacological trials, in comparison with the HEL 299 cell line, used as reference standard.

KW - In vitro culture

KW - MDCK cell line

KW - Pneumocystis carinii

UR - http://www.scopus.com/inward/record.url?scp=0030160950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030160950&partnerID=8YFLogxK

M3 - Article

C2 - 8758555

AN - SCOPUS:0030160950

VL - 3

SP - 183

EP - 185

JO - Parasite

JF - Parasite

SN - 1252-607X

IS - 2

ER -