Magnetic resonance (MR) imaging and MR spectroscopy of nerve regeneration and target muscle energy metabolism in a model of prosthesis-guided reinnervation in rats

Angela M. Baldassarri, Giorgio Zetti, Serge Masson, Stefano Gatti, Antonio Piazzini Albani, Gianfranco Ferla, Andrea C. Boicelli

Research output: Contribution to journalArticle

Abstract

Rationale and Objectives.: We monitored the regeneration of the rat sciatic nerve after its transection and the concomitant alteration in the high-energy phosphates content in the target tibialis anterior muscle. Methods.: Rat sciatic nerve was resected and the gap connected with a prosthesis of polytetrafluoroethylene. Progress of reinnervation was monitored by 1H MR imaging, whereas muscular energy metabolism was evaluated by localized 31P MR spectroscopy. Results.: Reconstitution between the nerve stumps was resumed 8-12 weeks postoperatively. The ratio of phosphocreatine to inorganic phosphate reached a plateau at 46% of the initial level approximately 8 weeks after the operation and recovered thereafter. Immediately after the surgery, muscular pH became slightly alkaline and returned to normal with the progress of reinnervation. Conclusion.: Recovery of the muscular energy metabolism began after the reconnection of the severed nerve stumps. The combination of MR imaging and MR spectroscopy followed noninvasively the progress of reinnervation and muscular energy metabolism of the prosthesis-guided nerve regeneration.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalAcademic Radiology
Volume2
Issue number2
DOIs
Publication statusPublished - 1995

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Nerve Regeneration
Energy Metabolism
Prostheses and Implants
Magnetic Resonance Spectroscopy
Magnetic Resonance Imaging
Sciatic Nerve
Muscles
Phosphates
Phosphocreatine
Polytetrafluoroethylene
Regeneration

Keywords

  • Denervation
  • magnetic resonance (MR) imaging
  • MR spectroscopy
  • muscle
  • rat

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Magnetic resonance (MR) imaging and MR spectroscopy of nerve regeneration and target muscle energy metabolism in a model of prosthesis-guided reinnervation in rats. / Baldassarri, Angela M.; Zetti, Giorgio; Masson, Serge; Gatti, Stefano; Albani, Antonio Piazzini; Ferla, Gianfranco; Boicelli, Andrea C.

In: Academic Radiology, Vol. 2, No. 2, 1995, p. 128-134.

Research output: Contribution to journalArticle

Baldassarri, Angela M. ; Zetti, Giorgio ; Masson, Serge ; Gatti, Stefano ; Albani, Antonio Piazzini ; Ferla, Gianfranco ; Boicelli, Andrea C. / Magnetic resonance (MR) imaging and MR spectroscopy of nerve regeneration and target muscle energy metabolism in a model of prosthesis-guided reinnervation in rats. In: Academic Radiology. 1995 ; Vol. 2, No. 2. pp. 128-134.
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AU - Gatti, Stefano

AU - Albani, Antonio Piazzini

AU - Ferla, Gianfranco

AU - Boicelli, Andrea C.

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AB - Rationale and Objectives.: We monitored the regeneration of the rat sciatic nerve after its transection and the concomitant alteration in the high-energy phosphates content in the target tibialis anterior muscle. Methods.: Rat sciatic nerve was resected and the gap connected with a prosthesis of polytetrafluoroethylene. Progress of reinnervation was monitored by 1H MR imaging, whereas muscular energy metabolism was evaluated by localized 31P MR spectroscopy. Results.: Reconstitution between the nerve stumps was resumed 8-12 weeks postoperatively. The ratio of phosphocreatine to inorganic phosphate reached a plateau at 46% of the initial level approximately 8 weeks after the operation and recovered thereafter. Immediately after the surgery, muscular pH became slightly alkaline and returned to normal with the progress of reinnervation. Conclusion.: Recovery of the muscular energy metabolism began after the reconnection of the severed nerve stumps. The combination of MR imaging and MR spectroscopy followed noninvasively the progress of reinnervation and muscular energy metabolism of the prosthesis-guided nerve regeneration.

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