Magnetic stirrer method for the detection of Trichinella larvae in muscle samples

Anne Mayer-Scholl, Edoardo Pozio, Jennifer Gayda, Nora Thaben, Peter Bahn, Karsten Nöckler

Research output: Contribution to journalArticlepeer-review

Abstract

Trichinellosis is a debilitating disease in humans and is caused by the consumption of raw or undercooked meat of animals infected with the nematode larvae of the genus Trichinella. The most important sources of human infections worldwide are game meat and pork or pork products. In many countries, the prevention of human trichinellosis is based on the identification of infected animals by means of the artificial digestion of muscle samples from susceptible animal carcasses. There are several methods based on the digestion of meat but the magnetic stirrer method is considered the gold standard. This method allows the detection of Trichinella larvae by microscopy after the enzymatic digestion of muscle samples and subsequent filtration and sedimentation steps. Although this method does not require special and expensive equipment, internal controls cannot be used. Therefore, stringent quality management should be applied throughout the test. The aim of the present work is to provide detailed handling instructions and critical control points of the method to analysts, based on the experience of the European Union Reference Laboratory for Parasites and the National Reference Laboratory of Germany for Trichinella.

Original languageEnglish
Article numbere55354
JournalJournal of Visualized Experiments
Volume2017
Issue number121
DOIs
Publication statusPublished - Mar 3 2017

Keywords

  • Digestion
  • Digestion method
  • Filtration
  • Food borne disease
  • Game meat
  • Infection
  • Issue 121
  • Magnetic stirrer method
  • Pork
  • Sedimentation
  • Trichinella larvae
  • Trichinellosis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'Magnetic stirrer method for the detection of Trichinella larvae in muscle samples'. Together they form a unique fingerprint.

Cite this