Maldi-TOF analysis of portal sera of pancreatic cancer patients: Identification of diabetogenic and antidiabetogenic peptides

Anna Valerio, Daniela Basso, Paola Fogar, Massimo Falconi, Eliana Greco, Claudio Bassi, Roberta Seraglia, Mohamed Abu-Hilal, Filippo Navaglia, Carlo Federico Zambon, Nicoletta Gallo, Alessandra Falda, Sergio Pedrazzoli, Mario Plebani

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Pancreatic cancer (PC) associated diabetes mellitus (DM) might be consequent to the diabetogenic effects of tumour products, possibly acting via nitric oxide (NO). Our aims were: (1) to verify whether PC associated DM determines an increased hepatic NO and (2) using MALDI-TOF analysis, to evaluate the peptide composition of PC cell conditioned media (CM) and of portal sera from patients with PC with (n=7) or without (n=4) DM. Methods: In liver tissue homogenates of 23 patients with PC (n=17) or chronic pancreatitis (n=6) GAPDH mRNA and activity, glucose, lactate, nitrite and nitrate were assayed. MALDI-TOF analysis was performed in three PC cell lines CM, and in portal sera from patients with PC. Results: Higher GAPDH mRNA and nitrite were found in patients with than in patients without DM. In PC cell CM, only 9 among a total of 75 fragments identified, were tumour specific. One hundred seventy-three fragments were identified in the portal sera of patients: one was positively and six fragments were negatively correlated with DM. Conclusions: Unlike liver GAPDH, NO appears to be involved in PC associated DM. In portal sera, the absence, rather than the presence, of specific fragments, appears to be correlated with the development of DM.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalClinica Chimica Acta
Volume343
Issue number1-2
DOIs
Publication statusPublished - May 2004

Keywords

  • Diabetes mellitus
  • Maldi-TOF
  • Nitric oxide
  • Pancreatic cancer

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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