Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells

Mirco Galiè, Carlo Sorrentino, Maura Montani, Luigi Micossi, Emma Di Carlo, Tommaso D'Antuono, Laura Calderan, Pasquina Marzola, Donatella Benati, Flavia Merigo, Fiorenza Orlando, Arianna Smorlesi, Cristina Marchini, Augusto Amici, Andrea Sbarbati

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The progression of a lesion to a carcinoma is dependent on the engagement of 'reactive stroma' that provides structural and vascular support for tumour growth and also leads to tissue reorganization and invasiveness. The composition of reactive stroma closely resembles that of granulation tissue, and myofibroblasts are thought to play a critical role in driving the stromal reaction of invasive tumours as well as of physiological wound repair. In the present work, we established a myofibroblast-like cell line, named A17, from a mouse mammary carcinoma model in which tumourigenesis is triggered in a single step by the overexpression of HER-2/neu transgene in the epithelial compartment of mammary glands. We showed that although they derived from a tumour of epithelial origin and did not express HER-2/neu transgene, their subcutaneous injection into the backs of syngeneic mice gave rise to sarcomatoid tumours which expressed alpha-smooth muscle actin at the invasive edge. The expression of cytokeratin 14 suggested a myoepithelial origin but immunophenotypical profile, invasive and neoangiogenic potential of A17 cells and tumours showed many similarities with the reactive stroma that occurs in wound repair and in cancerogenesis. Our results suggest that epithelial tumours have the potential to develop highly tumourigenic and invasive reactive stromal cells and our cell line represents a novel, effective model for studying epithelial-stromal interaction and the role of myofibroblasts in tumour development.

Original languageEnglish
Pages (from-to)1868-1878
Number of pages11
JournalCarcinogenesis
Volume26
Issue number11
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Stromal Cells
Breast Neoplasms
Myofibroblasts
Neoplasms
A 17
Transgenes
Keratin-14
Cell Line
Granulation Tissue
Wounds and Injuries
Subcutaneous Injections
Human Mammary Glands
Smooth Muscle
Blood Vessels
Actins
Carcinoma
Growth

ASJC Scopus subject areas

  • Cancer Research

Cite this

Galiè, M., Sorrentino, C., Montani, M., Micossi, L., Di Carlo, E., D'Antuono, T., ... Sbarbati, A. (2005). Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis, 26(11), 1868-1878. https://doi.org/10.1093/carcin/bgi158

Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. / Galiè, Mirco; Sorrentino, Carlo; Montani, Maura; Micossi, Luigi; Di Carlo, Emma; D'Antuono, Tommaso; Calderan, Laura; Marzola, Pasquina; Benati, Donatella; Merigo, Flavia; Orlando, Fiorenza; Smorlesi, Arianna; Marchini, Cristina; Amici, Augusto; Sbarbati, Andrea.

In: Carcinogenesis, Vol. 26, No. 11, 11.2005, p. 1868-1878.

Research output: Contribution to journalArticle

Galiè, M, Sorrentino, C, Montani, M, Micossi, L, Di Carlo, E, D'Antuono, T, Calderan, L, Marzola, P, Benati, D, Merigo, F, Orlando, F, Smorlesi, A, Marchini, C, Amici, A & Sbarbati, A 2005, 'Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells', Carcinogenesis, vol. 26, no. 11, pp. 1868-1878. https://doi.org/10.1093/carcin/bgi158
Galiè M, Sorrentino C, Montani M, Micossi L, Di Carlo E, D'Antuono T et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis. 2005 Nov;26(11):1868-1878. https://doi.org/10.1093/carcin/bgi158
Galiè, Mirco ; Sorrentino, Carlo ; Montani, Maura ; Micossi, Luigi ; Di Carlo, Emma ; D'Antuono, Tommaso ; Calderan, Laura ; Marzola, Pasquina ; Benati, Donatella ; Merigo, Flavia ; Orlando, Fiorenza ; Smorlesi, Arianna ; Marchini, Cristina ; Amici, Augusto ; Sbarbati, Andrea. / Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. In: Carcinogenesis. 2005 ; Vol. 26, No. 11. pp. 1868-1878.
@article{a526d7efcf5f43ee86f581acf70ef158,
title = "Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells",
abstract = "The progression of a lesion to a carcinoma is dependent on the engagement of 'reactive stroma' that provides structural and vascular support for tumour growth and also leads to tissue reorganization and invasiveness. The composition of reactive stroma closely resembles that of granulation tissue, and myofibroblasts are thought to play a critical role in driving the stromal reaction of invasive tumours as well as of physiological wound repair. In the present work, we established a myofibroblast-like cell line, named A17, from a mouse mammary carcinoma model in which tumourigenesis is triggered in a single step by the overexpression of HER-2/neu transgene in the epithelial compartment of mammary glands. We showed that although they derived from a tumour of epithelial origin and did not express HER-2/neu transgene, their subcutaneous injection into the backs of syngeneic mice gave rise to sarcomatoid tumours which expressed alpha-smooth muscle actin at the invasive edge. The expression of cytokeratin 14 suggested a myoepithelial origin but immunophenotypical profile, invasive and neoangiogenic potential of A17 cells and tumours showed many similarities with the reactive stroma that occurs in wound repair and in cancerogenesis. Our results suggest that epithelial tumours have the potential to develop highly tumourigenic and invasive reactive stromal cells and our cell line represents a novel, effective model for studying epithelial-stromal interaction and the role of myofibroblasts in tumour development.",
author = "Mirco Gali{\`e} and Carlo Sorrentino and Maura Montani and Luigi Micossi and {Di Carlo}, Emma and Tommaso D'Antuono and Laura Calderan and Pasquina Marzola and Donatella Benati and Flavia Merigo and Fiorenza Orlando and Arianna Smorlesi and Cristina Marchini and Augusto Amici and Andrea Sbarbati",
year = "2005",
month = "11",
doi = "10.1093/carcin/bgi158",
language = "English",
volume = "26",
pages = "1868--1878",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells

AU - Galiè, Mirco

AU - Sorrentino, Carlo

AU - Montani, Maura

AU - Micossi, Luigi

AU - Di Carlo, Emma

AU - D'Antuono, Tommaso

AU - Calderan, Laura

AU - Marzola, Pasquina

AU - Benati, Donatella

AU - Merigo, Flavia

AU - Orlando, Fiorenza

AU - Smorlesi, Arianna

AU - Marchini, Cristina

AU - Amici, Augusto

AU - Sbarbati, Andrea

PY - 2005/11

Y1 - 2005/11

N2 - The progression of a lesion to a carcinoma is dependent on the engagement of 'reactive stroma' that provides structural and vascular support for tumour growth and also leads to tissue reorganization and invasiveness. The composition of reactive stroma closely resembles that of granulation tissue, and myofibroblasts are thought to play a critical role in driving the stromal reaction of invasive tumours as well as of physiological wound repair. In the present work, we established a myofibroblast-like cell line, named A17, from a mouse mammary carcinoma model in which tumourigenesis is triggered in a single step by the overexpression of HER-2/neu transgene in the epithelial compartment of mammary glands. We showed that although they derived from a tumour of epithelial origin and did not express HER-2/neu transgene, their subcutaneous injection into the backs of syngeneic mice gave rise to sarcomatoid tumours which expressed alpha-smooth muscle actin at the invasive edge. The expression of cytokeratin 14 suggested a myoepithelial origin but immunophenotypical profile, invasive and neoangiogenic potential of A17 cells and tumours showed many similarities with the reactive stroma that occurs in wound repair and in cancerogenesis. Our results suggest that epithelial tumours have the potential to develop highly tumourigenic and invasive reactive stromal cells and our cell line represents a novel, effective model for studying epithelial-stromal interaction and the role of myofibroblasts in tumour development.

AB - The progression of a lesion to a carcinoma is dependent on the engagement of 'reactive stroma' that provides structural and vascular support for tumour growth and also leads to tissue reorganization and invasiveness. The composition of reactive stroma closely resembles that of granulation tissue, and myofibroblasts are thought to play a critical role in driving the stromal reaction of invasive tumours as well as of physiological wound repair. In the present work, we established a myofibroblast-like cell line, named A17, from a mouse mammary carcinoma model in which tumourigenesis is triggered in a single step by the overexpression of HER-2/neu transgene in the epithelial compartment of mammary glands. We showed that although they derived from a tumour of epithelial origin and did not express HER-2/neu transgene, their subcutaneous injection into the backs of syngeneic mice gave rise to sarcomatoid tumours which expressed alpha-smooth muscle actin at the invasive edge. The expression of cytokeratin 14 suggested a myoepithelial origin but immunophenotypical profile, invasive and neoangiogenic potential of A17 cells and tumours showed many similarities with the reactive stroma that occurs in wound repair and in cancerogenesis. Our results suggest that epithelial tumours have the potential to develop highly tumourigenic and invasive reactive stromal cells and our cell line represents a novel, effective model for studying epithelial-stromal interaction and the role of myofibroblasts in tumour development.

UR - http://www.scopus.com/inward/record.url?scp=27744484362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744484362&partnerID=8YFLogxK

U2 - 10.1093/carcin/bgi158

DO - 10.1093/carcin/bgi158

M3 - Article

C2 - 15975963

AN - SCOPUS:27744484362

VL - 26

SP - 1868

EP - 1878

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 11

ER -