Mammosphere-forming cells from breast cancer cell lines as a tool for the identification of CSC-like- and early progenitor-targeting drugs

Mario Cioce, Simona Gherardi, Giuseppe Viglietto, Sabrina Strano, Giovanni Blandino, Paola Muti, Gennaro Ciliberto

Research output: Contribution to journalArticle

Abstract

Here we show that a subpopulation of MCF-7 breast cancer cells which stains pale to toluidine Blue (Light Cells- LCs), is endowed with features of CSCs. LCs give rise to self-renewing mammospheres and express typical CSC markers; moreover this subpopulation is chemoresistant and highly tumorigenic in vivo. LCs can be identified in several other breast cancer cell lines, irrespectively of their histological origin (luminal vs. mesenchymal vs. basal) and represent an heterogeneous cell population composed mainly of CSC-like and early progenitor cells. By a limited in vitro drug screening assay, we identify compounds which can specifically interfere with the viability of LCs from multiple breast cancer cell lines. Analysis of the Sphere-Forming efficiency (SFe) and of the distribution of ALDHbright cells within the treated cell lines suggest that one of the identified compounds acts in vitro by modulating the CSC phenotype. Interestingly, a subset of the identified compounds is known to affect directly or indirectly the NFκB pathway which is emerging as an important modulator of CSC proliferation and chemoresistance.

Original languageEnglish
Pages (from-to)2878-2887
Number of pages10
JournalCell Cycle
Volume9
Issue number14
DOIs
Publication statusPublished - Jul 15 2010

Keywords

  • ALDH
  • Breast cancer
  • CD49f
  • Mammospheres
  • MCF-7
  • NfκB
  • Toluidine blue

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

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