Manipulation of the growth rate of human breast cancer cells by antiandrogen followed by chemotherapy

A. Alama, L. Miglietta, R. E. Favoni, F. Barbieri, F. Ravera, L. Fazzuoli, G. Nicolo, A. Nicolin

Research output: Contribution to journalArticlepeer-review

Abstract

The effects induced by the antiandrogen Cyproterone Acetate (CPA) on the proliferation of EVSA-T human breast cancer cells endowed with androgen receptors were studied. Kinetic analyses were carried out by two autoradiographic techniques measuring the percentage of cells in S-phase and the growth fraction (GF). The exposure of the cultures to CPA for 24 h caused a marked inhibition on S-phase cells without significantly affecting the GF. The accumulation of cells in G1-phase, confirmed by cytometric analysis, was rescued to the S compartment by replacing the culture medium 24 h after CPA administration. Exposure of EVSA-T to Doxorubicin or Methotrexate after CPA and medium change at the time of maximal proliferative recruitment, led to an enhancement of cytotoxicity as demonstrated by colony survival assay.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalAnticancer Research
Volume10
Issue number3
Publication statusPublished - 1990

Keywords

  • Antiandrogen
  • Breast cancer
  • Cell kinetics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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