Mannose-binding lectin-2 genetic variation and stomach cancer risk

Andrea Baccarelli, Lifang Hou, Jinbo Chen, Jolanta Lissowska, Emad M. El-Omar, Paolo Grillo, Sara M. Giacomini, Meredith Yaeger, Toralf Bernig, Witold Zatonski, Joseph F. Fraumeni, Stephen J. Chanock, Wong Ho Chow

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Abstract

Deficiency of the mannose-binding lectin (MBL) protein, an antigen-recognition molecule involved in systemic and mucosal innate immunity, is determined by variant alleles in MBL2 gene promoter and exon-1 regions. We conducted a population-based study on 305 stomach cancer cases and 427 controls in Warsaw, Poland to determine whether MBL2 gene variants predispose to stomach cancer. Single nucleotide polymorphisms (SNPs) in MBL2 were determined by TaqMan™. The 5 tested MBL2 variants are in complete linkage disequilibrium and comprise 6 different haplotypes. The risk of stomach cancer was increased in subjects carrying the H/H promoter genotype (OR = 1.8, 95% CI 1.1-2.9; p = 0.020) relative to L/L carriers, after adjustment for age, gender, education and smoking. Carrying at least one D exon-1 allele was associated with nonsignificant excess risk (OR = 1.5, 95% CI 0.9-2.4; p = 0.081). In haplotype analysis, the HYD haplotype was associated with increased risk of stomach cancer when compared with HYA, the most common haplotype (OR = 1.9, 95% CI 1.1-3.2; p = 0.021). In diplotype analysis, subjects carrying the YA/D haplotype combination showed the highest risk (OR = 3.0, 95% CI 1.2-7.1; p = 0.015), compared with YA/YA. Further analyses to examine the joint effect of MBL2 and IL-1B polymorphisms, previously shown to predispose to stomach cancer, indicated that the combination of at-risk IL-1B genotypes (CT or TT at location -511) and HYD MBL2 haplotype was associated with a 3.5-fold risk (OR = 3.5, 95% CI 1.6-7.6; p = 0.001). Our findings suggest that the codon 52 D MBL2 variant causing a cysteine > arginine replacement, but not B and C variants producing glycine substitutions, is specifically associated with gastric cancer risk.

Original languageEnglish
Pages (from-to)1970-1975
Number of pages6
JournalInternational Journal of Cancer
Volume119
Issue number8
DOIs
Publication statusPublished - Oct 15 2006

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Mannose-Binding Lectin
Stomach Neoplasms
Haplotypes
Exons
Alleles
Genotype
Mucosal Immunity
Linkage Disequilibrium
Poland
Innate Immunity
Codon
Glycine
Genes
Single Nucleotide Polymorphism
Cysteine
Arginine
Smoking
Education
Antigens

Keywords

  • Haplotypes
  • Immunologic deficiency syndromes
  • Mannose-binding lectin
  • Stomach cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Baccarelli, A., Hou, L., Chen, J., Lissowska, J., El-Omar, E. M., Grillo, P., ... Chow, W. H. (2006). Mannose-binding lectin-2 genetic variation and stomach cancer risk. International Journal of Cancer, 119(8), 1970-1975. https://doi.org/10.1002/ijc.22075

Mannose-binding lectin-2 genetic variation and stomach cancer risk. / Baccarelli, Andrea; Hou, Lifang; Chen, Jinbo; Lissowska, Jolanta; El-Omar, Emad M.; Grillo, Paolo; Giacomini, Sara M.; Yaeger, Meredith; Bernig, Toralf; Zatonski, Witold; Fraumeni, Joseph F.; Chanock, Stephen J.; Chow, Wong Ho.

In: International Journal of Cancer, Vol. 119, No. 8, 15.10.2006, p. 1970-1975.

Research output: Contribution to journalArticle

Baccarelli, A, Hou, L, Chen, J, Lissowska, J, El-Omar, EM, Grillo, P, Giacomini, SM, Yaeger, M, Bernig, T, Zatonski, W, Fraumeni, JF, Chanock, SJ & Chow, WH 2006, 'Mannose-binding lectin-2 genetic variation and stomach cancer risk', International Journal of Cancer, vol. 119, no. 8, pp. 1970-1975. https://doi.org/10.1002/ijc.22075
Baccarelli A, Hou L, Chen J, Lissowska J, El-Omar EM, Grillo P et al. Mannose-binding lectin-2 genetic variation and stomach cancer risk. International Journal of Cancer. 2006 Oct 15;119(8):1970-1975. https://doi.org/10.1002/ijc.22075
Baccarelli, Andrea ; Hou, Lifang ; Chen, Jinbo ; Lissowska, Jolanta ; El-Omar, Emad M. ; Grillo, Paolo ; Giacomini, Sara M. ; Yaeger, Meredith ; Bernig, Toralf ; Zatonski, Witold ; Fraumeni, Joseph F. ; Chanock, Stephen J. ; Chow, Wong Ho. / Mannose-binding lectin-2 genetic variation and stomach cancer risk. In: International Journal of Cancer. 2006 ; Vol. 119, No. 8. pp. 1970-1975.
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