Mapping of a potent transcriptional repression region of the human homeodomain protein EVX1

Paola Briata, Cristina Ilengo, Rinke Van Dewerken, Giorgio Corte

Research output: Contribution to journalArticle

Abstract

The human homeodomain protein EVX1 is a transcriptional repressor in transfected mammalian cells and this function depends on a region carboxyl-terminal to the homeodomain. In this study, we transiently expressed several deletions of the EVX1 C-terminal region in mammalian cells and investigated their effect on the transcription of a reporter gene directed by different promoters. We show that the repressor activity maps to a region of 51 amino acids with a high abundance of alanine and proline residues. This region is able to transfer the repressor function to either the entire HOXC6 or CREB transcription factors, or to the GAL4 DNA binding domain.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalFEBS Letters
Volume402
Issue number2-3
DOIs
Publication statusPublished - Jan 27 1997

Keywords

  • EVX1
  • Gene transcription
  • Repression domain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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