Mapping of chromosome 17 breakpoints in acute myeloid leukemias

Letizia Longo, Emilio Donti, Amedea Mencarelli, Giancarlo Avanzi, Luigi Pegoraro, Giuliana Alimena, Antonio Tabilio, Giovanna Venti, Fausto Grignani, Pier Giuseppe Pelicci

Research output: Contribution to journalArticlepeer-review

Abstract

The 17q11-21 chromosomal region is frequently involved in non-random structural rearrangements associated with the M1 and M2 subtypes of acute myeloid leukemias (AML), as well as with the 15;17 translocation typical of the promyelocytic subtype. A number of genes have been localized in this region including the c-erbA-1 and c-erbB-2 proto-oncogenes, the genes coding for the granulocytecolony stimulating factor (G-CSF), the retinoic acid receptor α (RARα) and the myeloperoxidase enzyme (MPO). However, the precise location of these genes in relationship to the 17q11-21 breakpoint(s) has not been determined. Using in situ hybridization on metaphase chromosomes, we established the position of the break-points in relationship to the c-erbA-1, c-erbB-2, G-CSF, RARα and MPO loci in a series of AML cases bearing 17q11-21 rearrangements. We report: (i) that the respective position of the five genes is centromere - c-erbA-1-G-CSF - c-erbB-2 - RARα - MPO - telomere; (ii) that the breakpoints of the various AML subtypes are variably located between the centromere and c-erbB-2 in M1 and M2; (iii) that the breakpoints are consistently located between c-erbB-2 and RARα/MPO in M3; and (iv) that the breakpoint on chromosome 17 in the 15;17 translocation is located on 17q21 and not on 17q11-12 as previously reported.

Original languageEnglish
Pages (from-to)1557-1563
Number of pages7
JournalOncogene
Volume5
Issue number10
Publication statusPublished - Oct 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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