Mapping of mitochondrial metabolic competence by cytochrome oxidase and succinic dehydrogenase cytochemistry

C. Bertoni-Freddari, P. Fattoretti, T. Casoli, G. Di Stefano, M. Solazzi, N. Gracciotti, P. Pompei

Research output: Contribution to journalArticlepeer-review

Abstract

To map the mitochondrial capacity to provide adenosine triphosphate (ATP), the activities of cytochrome oxidase (COX) and succinic dehydrogenase (SDH) were respectively evidenced by diaminobenzidine (DAB) and copper ferrocyanide cytochemical techniques in the cerebellar cortex of adult rats. Sampling of the positive mitochondria was carried out by the disector procedure. The ratio (R) overall area of the precipitates due to COX activity within the single mitochondrion/area of the same organelle was automatically calculated to estimate enzyme activity vs mitochondrial size. The number of SDH-positive mitochondria/μm3 of tissue (numeric density, Nv) was morphometrically calculated. Cytochemistry of key enzymes of the respiratory chain enables measurement of the actual capacity of individual mitochondria to provide ATP. This quantitative estimation allows morphofunctional mapping of the mitochondrial metabolic competence in discrete tissue and/or cellular compartments.

Original languageEnglish
Pages (from-to)1191-1192
Number of pages2
JournalJournal of Histochemistry and Cytochemistry
Volume49
Issue number9
Publication statusPublished - 2001

Keywords

  • Adenosine triphosphate
  • Cytochemistry
  • Cytochrome oxidase
  • Mitochondrial mapping
  • Succinic dehydrogenase

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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