Abstract
To map the mitochondrial capacity to provide adenosine triphosphate (ATP), the activities of cytochrome oxidase (COX) and succinic dehydrogenase (SDH) were respectively evidenced by diaminobenzidine (DAB) and copper ferrocyanide cytochemical techniques in the cerebellar cortex of adult rats. Sampling of the positive mitochondria was carried out by the disector procedure. The ratio (R) overall area of the precipitates due to COX activity within the single mitochondrion/area of the same organelle was automatically calculated to estimate enzyme activity vs mitochondrial size. The number of SDH-positive mitochondria/μm3 of tissue (numeric density, Nv) was morphometrically calculated. Cytochemistry of key enzymes of the respiratory chain enables measurement of the actual capacity of individual mitochondria to provide ATP. This quantitative estimation allows morphofunctional mapping of the mitochondrial metabolic competence in discrete tissue and/or cellular compartments.
| Original language | English |
|---|---|
| Pages (from-to) | 1191-1192 |
| Number of pages | 2 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 49 |
| Issue number | 9 |
| Publication status | Published - 2001 |
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Keywords
- Adenosine triphosphate
- Cytochemistry
- Cytochrome oxidase
- Mitochondrial mapping
- Succinic dehydrogenase
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Mapping of mitochondrial metabolic competence by cytochrome oxidase and succinic dehydrogenase cytochemistry. / Bertoni-Freddari, C.; Fattoretti, P.; Casoli, T.; Di Stefano, G.; Solazzi, M.; Gracciotti, N.; Pompei, P.
In: Journal of Histochemistry and Cytochemistry, Vol. 49, No. 9, 2001, p. 1191-1192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mapping of mitochondrial metabolic competence by cytochrome oxidase and succinic dehydrogenase cytochemistry
AU - Bertoni-Freddari, C.
AU - Fattoretti, P.
AU - Casoli, T.
AU - Di Stefano, G.
AU - Solazzi, M.
AU - Gracciotti, N.
AU - Pompei, P.
PY - 2001
Y1 - 2001
N2 - To map the mitochondrial capacity to provide adenosine triphosphate (ATP), the activities of cytochrome oxidase (COX) and succinic dehydrogenase (SDH) were respectively evidenced by diaminobenzidine (DAB) and copper ferrocyanide cytochemical techniques in the cerebellar cortex of adult rats. Sampling of the positive mitochondria was carried out by the disector procedure. The ratio (R) overall area of the precipitates due to COX activity within the single mitochondrion/area of the same organelle was automatically calculated to estimate enzyme activity vs mitochondrial size. The number of SDH-positive mitochondria/μm3 of tissue (numeric density, Nv) was morphometrically calculated. Cytochemistry of key enzymes of the respiratory chain enables measurement of the actual capacity of individual mitochondria to provide ATP. This quantitative estimation allows morphofunctional mapping of the mitochondrial metabolic competence in discrete tissue and/or cellular compartments.
AB - To map the mitochondrial capacity to provide adenosine triphosphate (ATP), the activities of cytochrome oxidase (COX) and succinic dehydrogenase (SDH) were respectively evidenced by diaminobenzidine (DAB) and copper ferrocyanide cytochemical techniques in the cerebellar cortex of adult rats. Sampling of the positive mitochondria was carried out by the disector procedure. The ratio (R) overall area of the precipitates due to COX activity within the single mitochondrion/area of the same organelle was automatically calculated to estimate enzyme activity vs mitochondrial size. The number of SDH-positive mitochondria/μm3 of tissue (numeric density, Nv) was morphometrically calculated. Cytochemistry of key enzymes of the respiratory chain enables measurement of the actual capacity of individual mitochondria to provide ATP. This quantitative estimation allows morphofunctional mapping of the mitochondrial metabolic competence in discrete tissue and/or cellular compartments.
KW - Adenosine triphosphate
KW - Cytochemistry
KW - Cytochrome oxidase
KW - Mitochondrial mapping
KW - Succinic dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0034868747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034868747&partnerID=8YFLogxK
M3 - Article
C2 - 11511690
AN - SCOPUS:0034868747
VL - 49
SP - 1191
EP - 1192
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 9
ER -