TY - JOUR
T1 - Mapping of the molecular determinants involved in the interaction between eps15 and AP-2
AU - Iannolo, Gioacchin
AU - Salcini, Anna Elisabetta
AU - Gaidarov, Ibragim
AU - Goodman, Oscar B.
AU - Baulida, Josep
AU - Carpenter, Graham
AU - Pelicci, Pier Giuseppe
AU - Di Fiore, Pier Paolo
AU - Keen, James H.
PY - 1997
Y1 - 1997
N2 - eps15, a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases, possesses a discrete domain structure with protein-binding properties. It interacts with a number of cellular proteins through an evolutionarily conserved protein-binding domain, the eps15 homology domain, located in it NH2-terminal region. In addition, a proline- rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans ~80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved in the binding. AP-2 binds to eps15 through its α subunit (α- adaptin); in particular, the COOH-terminal region of α-adaptin, the so- called α-ear, contains the eps15 binding region.
AB - eps15, a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases, possesses a discrete domain structure with protein-binding properties. It interacts with a number of cellular proteins through an evolutionarily conserved protein-binding domain, the eps15 homology domain, located in it NH2-terminal region. In addition, a proline- rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans ~80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved in the binding. AP-2 binds to eps15 through its α subunit (α- adaptin); in particular, the COOH-terminal region of α-adaptin, the so- called α-ear, contains the eps15 binding region.
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M3 - Article
C2 - 9000562
AN - SCOPUS:0031032314
VL - 57
SP - 240
EP - 245
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 2
ER -