Mapping polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin

Valerio Orlando, Renato Paro

Research output: Contribution to journalArticle

285 Citations (Scopus)

Abstract

The Polycomb group (Pc-G) proteins are responsible for keeping developmental regulators, like homeotic genes, stably and inheritably repressed during Drosophila development. Several similarities to a protein class involved in heterochromatin formation suggest that the Pc-G exerts its function at the higher order chromatin level. Here we have mapped the distribution of the Pc protein in the homeotic bithorax complex (BX-C) of Drosophila tissue culture cells. We have elaborated a method, based on the in vivo formaldehyde cross-linking technique, that allows a substantial enrichment for Pc-interacting sites by immunoprecipitation of the cross-linked chromatin with anti-Pc antibodies. We find that the Pc protein quantitatively covers large regulatory regions of repressed BX-C genes. Conversely, we find that the Abdominal-B gene is active in these cells and the region devold of any bound Pc protein.

Original languageEnglish
Pages (from-to)1187-1198
Number of pages12
JournalCell
Volume75
Issue number6
DOIs
Publication statusPublished - Dec 17 1993

Fingerprint

Formaldehyde
Chromatin
Drosophila
Genes
Polycomb-Group Proteins
Homeodomain Proteins
Proteins
Heterochromatin
Homeobox Genes
Nucleic Acid Regulatory Sequences
Immunoprecipitation
Tissue culture
Anti-Idiotypic Antibodies
Cell Culture Techniques
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Mapping polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin. / Orlando, Valerio; Paro, Renato.

In: Cell, Vol. 75, No. 6, 17.12.1993, p. 1187-1198.

Research output: Contribution to journalArticle

Orlando, Valerio ; Paro, Renato. / Mapping polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin. In: Cell. 1993 ; Vol. 75, No. 6. pp. 1187-1198.
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