Mapping protein matrix cavities in human cytoglobin through Xe atom binding

Daniele De Sanctis, Sylvia Dewilde, Alessandra Pesce, Luc Moens, Paolo Ascenzi, Thomas Hankeln, Thorsten Burmester, Martino Bolognesi

Research output: Contribution to journalArticle

Abstract

Cytoglobin is the fourth recognized globin type, almost ubiquitously distributed in human tissues; its function is still poorly understood. Cytoglobin displays a core region of about 150 residues, structurally related to hemoglobin and myoglobin, and two extra segments, about 20 residues each, at the N- and C-termini. The core region hosts a large apolar cavity, held to provide a ligand diffusion pathway to/from the heme, and/or ligand temporary docking sites. Here we report the crystal structure (2.4Å resolution, R-factor 19.1%) of a human cytoglobin mutant bearing the CysB2(38)→Ser and CysE9(83)→Ser substitutions (CYGB*), treated under pressurized xenon. Three Xe atoms bind to the heme distal site region of CYGB* mapping the protein matrix apolar cavity. Despite the conserved globin fold, the cavity found in CYGB* is structured differently from those recognized to play a functional role in myoglobin, neuroglobin, truncated hemoglobins, and Cerebratulus lacteus mini-hemoglobin.

Original languageEnglish
Pages (from-to)1217-1221
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number4
DOIs
Publication statusPublished - Apr 16 2004

Fingerprint

Globins
Myoglobin
Heme
Atoms
Truncated Hemoglobins
R388
Hemoglobins
Bearings (structural)
Ligands
Xenon
Proteins
Substitution reactions
Crystal structure
Tissue
cytoglobin
neuroglobin

Keywords

  • Heme ligand diffusion
  • Human cytoglobin
  • Protein matrix cavities/tunnels
  • X-ray crystallography
  • Xe binding

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

De Sanctis, D., Dewilde, S., Pesce, A., Moens, L., Ascenzi, P., Hankeln, T., ... Bolognesi, M. (2004). Mapping protein matrix cavities in human cytoglobin through Xe atom binding. Biochemical and Biophysical Research Communications, 316(4), 1217-1221. https://doi.org/10.1016/j.bbrc.2004.03.007

Mapping protein matrix cavities in human cytoglobin through Xe atom binding. / De Sanctis, Daniele; Dewilde, Sylvia; Pesce, Alessandra; Moens, Luc; Ascenzi, Paolo; Hankeln, Thomas; Burmester, Thorsten; Bolognesi, Martino.

In: Biochemical and Biophysical Research Communications, Vol. 316, No. 4, 16.04.2004, p. 1217-1221.

Research output: Contribution to journalArticle

De Sanctis, D, Dewilde, S, Pesce, A, Moens, L, Ascenzi, P, Hankeln, T, Burmester, T & Bolognesi, M 2004, 'Mapping protein matrix cavities in human cytoglobin through Xe atom binding', Biochemical and Biophysical Research Communications, vol. 316, no. 4, pp. 1217-1221. https://doi.org/10.1016/j.bbrc.2004.03.007
De Sanctis, Daniele ; Dewilde, Sylvia ; Pesce, Alessandra ; Moens, Luc ; Ascenzi, Paolo ; Hankeln, Thomas ; Burmester, Thorsten ; Bolognesi, Martino. / Mapping protein matrix cavities in human cytoglobin through Xe atom binding. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 316, No. 4. pp. 1217-1221.
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