Mapping the function of polycomb proteins

Research output: Contribution to journalArticlepeer-review

Abstract

Polycomb group (PcG) proteins are master regulators of proliferation and development that play essential roles in human pathologies including cancers. PcGs act as gatekeepers of cellular identity, maintaining repression of a multitude of target genes. However, these properties have only been recently uncovered thanks to technological advances, first of all chromatin immunoprecipitations (ChIP), that allowed a systematic characterization of the activity of these factors in an unbiased manner at a genome-wide level. Using PcG protein as example, this chapter introduces the readers to the use of chromatin analysis (ChIP assays and replication timing) and how to move these approaches to a level of genome-wide interpretation.

Original languageEnglish
Pages (from-to)3-6
Number of pages4
JournalMethods in Molecular Biology
Volume1480
DOIs
Publication statusPublished - 2016

Keywords

  • ChIP
  • Chromatin
  • Next-generation sequencing
  • Polycomb
  • Replication

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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