Abstract
Objectives: To link local proinflammatory cytokines with HIV related nucleic acids in cervico-vaginal secretions and the factors associated with them. Methods: An observational study on 60 HIV positive women attending the department of obstetrics and gynaecology, University of Pavia, Italy. HIV-1 RNA in plasma, proviral HIV-1-DNA, cell associated and cell free HIV-1 RNA in cervico-vaginal secretions were evaluated by competitive polymerase chain reaction (c-PCR) and reverse transcriptase PCR (cRT-PCR). IL-1β, IL-6, and TNF-α were measured by ELISA in cervico-vaginal lavages. Multiple regression analysis on ordinal categorical variables was used to test for the simultaneous associations of clinical and microbiological variables on quartiles of cytokine concentrations in lavage samples. Results: Proviral HIV-1 DNA, cell associated and cell free HIV-1 RNA were detected in 76.7% (46/60), 70% (42/60), and 71.7% (43/60) of the patients, respectively. IL-1β concentration was directly correlated with proviral HIV-DNA (Spearman rho = 0.35, p = 0.01) and cell associated HIV-RNA levels (Spearman rho = 0.263, p = 0.05). IL-1β concentration (153.9 pg/ml) was higher (p
| Original language | English |
|---|---|
| Pages (from-to) | 108-112 |
| Number of pages | 5 |
| Journal | Sexually Transmitted Infections |
| Volume | 80 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 2004 |
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ASJC Scopus subject areas
- Dermatology
- Microbiology (medical)
- Immunology
Cite this
Markers of local immunity in cervico-vaginal secretions of HIV infected women : Implications for HIV shedding. / Zara, F.; Nappi, R. E.; Brerra, R.; Migliavacca, R.; Maserati, R.; Spinillo, A.
In: Sexually Transmitted Infections, Vol. 80, No. 2, 04.2004, p. 108-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Markers of local immunity in cervico-vaginal secretions of HIV infected women
T2 - Implications for HIV shedding
AU - Zara, F.
AU - Nappi, R. E.
AU - Brerra, R.
AU - Migliavacca, R.
AU - Maserati, R.
AU - Spinillo, A.
PY - 2004/4
Y1 - 2004/4
N2 - Objectives: To link local proinflammatory cytokines with HIV related nucleic acids in cervico-vaginal secretions and the factors associated with them. Methods: An observational study on 60 HIV positive women attending the department of obstetrics and gynaecology, University of Pavia, Italy. HIV-1 RNA in plasma, proviral HIV-1-DNA, cell associated and cell free HIV-1 RNA in cervico-vaginal secretions were evaluated by competitive polymerase chain reaction (c-PCR) and reverse transcriptase PCR (cRT-PCR). IL-1β, IL-6, and TNF-α were measured by ELISA in cervico-vaginal lavages. Multiple regression analysis on ordinal categorical variables was used to test for the simultaneous associations of clinical and microbiological variables on quartiles of cytokine concentrations in lavage samples. Results: Proviral HIV-1 DNA, cell associated and cell free HIV-1 RNA were detected in 76.7% (46/60), 70% (42/60), and 71.7% (43/60) of the patients, respectively. IL-1β concentration was directly correlated with proviral HIV-DNA (Spearman rho = 0.35, p = 0.01) and cell associated HIV-RNA levels (Spearman rho = 0.263, p = 0.05). IL-1β concentration (153.9 pg/ml) was higher (p
AB - Objectives: To link local proinflammatory cytokines with HIV related nucleic acids in cervico-vaginal secretions and the factors associated with them. Methods: An observational study on 60 HIV positive women attending the department of obstetrics and gynaecology, University of Pavia, Italy. HIV-1 RNA in plasma, proviral HIV-1-DNA, cell associated and cell free HIV-1 RNA in cervico-vaginal secretions were evaluated by competitive polymerase chain reaction (c-PCR) and reverse transcriptase PCR (cRT-PCR). IL-1β, IL-6, and TNF-α were measured by ELISA in cervico-vaginal lavages. Multiple regression analysis on ordinal categorical variables was used to test for the simultaneous associations of clinical and microbiological variables on quartiles of cytokine concentrations in lavage samples. Results: Proviral HIV-1 DNA, cell associated and cell free HIV-1 RNA were detected in 76.7% (46/60), 70% (42/60), and 71.7% (43/60) of the patients, respectively. IL-1β concentration was directly correlated with proviral HIV-DNA (Spearman rho = 0.35, p = 0.01) and cell associated HIV-RNA levels (Spearman rho = 0.263, p = 0.05). IL-1β concentration (153.9 pg/ml) was higher (p
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U2 - 10.1136/sti.2003.005157
DO - 10.1136/sti.2003.005157
M3 - Article
VL - 80
SP - 108
EP - 112
JO - Sexually Transmitted Infections
JF - Sexually Transmitted Infections
SN - 1368-4973
IS - 2
ER -