Markers of local immunity in cervico-vaginal secretions of HIV infected women

Implications for HIV shedding

F. Zara, R. E. Nappi, R. Brerra, R. Migliavacca, R. Maserati, A. Spinillo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objectives: To link local proinflammatory cytokines with HIV related nucleic acids in cervico-vaginal secretions and the factors associated with them. Methods: An observational study on 60 HIV positive women attending the department of obstetrics and gynaecology, University of Pavia, Italy. HIV-1 RNA in plasma, proviral HIV-1-DNA, cell associated and cell free HIV-1 RNA in cervico-vaginal secretions were evaluated by competitive polymerase chain reaction (c-PCR) and reverse transcriptase PCR (cRT-PCR). IL-1β, IL-6, and TNF-α were measured by ELISA in cervico-vaginal lavages. Multiple regression analysis on ordinal categorical variables was used to test for the simultaneous associations of clinical and microbiological variables on quartiles of cytokine concentrations in lavage samples. Results: Proviral HIV-1 DNA, cell associated and cell free HIV-1 RNA were detected in 76.7% (46/60), 70% (42/60), and 71.7% (43/60) of the patients, respectively. IL-1β concentration was directly correlated with proviral HIV-DNA (Spearman rho = 0.35, p = 0.01) and cell associated HIV-RNA levels (Spearman rho = 0.263, p = 0.05). IL-1β concentration (153.9 pg/ml) was higher (p

Original languageEnglish
Pages (from-to)108-112
Number of pages5
JournalSexually Transmitted Infections
Volume80
Issue number2
DOIs
Publication statusPublished - Apr 2004

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HIV-1
Immunity
HIV
Interleukin-1
RNA
DNA
Vaginal Douching
Cytokines
Hospital Obstetrics and Gynecology Department
Therapeutic Irrigation
Reverse Transcriptase Polymerase Chain Reaction
Gynecology
Nucleic Acids
Italy
Observational Studies
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Dermatology
  • Microbiology (medical)
  • Immunology

Cite this

Markers of local immunity in cervico-vaginal secretions of HIV infected women : Implications for HIV shedding. / Zara, F.; Nappi, R. E.; Brerra, R.; Migliavacca, R.; Maserati, R.; Spinillo, A.

In: Sexually Transmitted Infections, Vol. 80, No. 2, 04.2004, p. 108-112.

Research output: Contribution to journalArticle

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