Mass spectrometric identification and analysis of some aphidicolin metabolites in cancer patients

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We used gas chromatography/mass spectrometry to identify and quantitate some aphidicolin metabelites in plasma and urine of patients receiving aphidicolin-17-glycinate. The major metabolite found in plasma was 3-ketoaphidicolin, present at about one thent the concentrations of aphidicolin. 3-Ketoaphidicolin undergoes dehydroxymethylation to give 18-nor-3-ketoaphidicolin. This metabolite was also found in plasma and its concentration reached a maximum of 1% of aphidicolin. Small amounts of aphidicolin and 3-ketoaphidicolin were found free in urine but almost all of the drug was conjugated to glucuronic acid, as shown by mass spectrometric analysis of urine extracts and by enzymatic digestion with β-glucoronidase followed by gas chromatographic/mass spectrometric analysis.

Original languageEnglish
Pages (from-to)351-357
Number of pages7
JournalBiological Mass Spectrometry
Volume22
Issue number6
DOIs
Publication statusPublished - Jun 1993

Fingerprint

Aphidicolin
Metabolites
Urine
Plasmas
Neoplasms
Glucuronic Acid
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Digestion
Gases
Pharmaceutical Preparations
3-ketoaphidicolin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Mass spectrometric identification and analysis of some aphidicolin metabolites in cancer patients. / Davoli, E.; Zucchetti, M.; D'Incalci, M.; Sessa, C.; Cavalli, F.; Fanelli, R.

In: Biological Mass Spectrometry, Vol. 22, No. 6, 06.1993, p. 351-357.

Research output: Contribution to journalArticle

@article{82be097fb038478b8e874cbbb42806fa,
title = "Mass spectrometric identification and analysis of some aphidicolin metabolites in cancer patients",
abstract = "We used gas chromatography/mass spectrometry to identify and quantitate some aphidicolin metabelites in plasma and urine of patients receiving aphidicolin-17-glycinate. The major metabolite found in plasma was 3-ketoaphidicolin, present at about one thent the concentrations of aphidicolin. 3-Ketoaphidicolin undergoes dehydroxymethylation to give 18-nor-3-ketoaphidicolin. This metabolite was also found in plasma and its concentration reached a maximum of 1{\%} of aphidicolin. Small amounts of aphidicolin and 3-ketoaphidicolin were found free in urine but almost all of the drug was conjugated to glucuronic acid, as shown by mass spectrometric analysis of urine extracts and by enzymatic digestion with β-glucoronidase followed by gas chromatographic/mass spectrometric analysis.",
author = "E. Davoli and M. Zucchetti and M. D'Incalci and C. Sessa and F. Cavalli and R. Fanelli",
year = "1993",
month = "6",
doi = "10.1002/bms.1200220607",
language = "English",
volume = "22",
pages = "351--357",
journal = "Biological Mass Spectrometry",
issn = "1052-9306",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Mass spectrometric identification and analysis of some aphidicolin metabolites in cancer patients

AU - Davoli, E.

AU - Zucchetti, M.

AU - D'Incalci, M.

AU - Sessa, C.

AU - Cavalli, F.

AU - Fanelli, R.

PY - 1993/6

Y1 - 1993/6

N2 - We used gas chromatography/mass spectrometry to identify and quantitate some aphidicolin metabelites in plasma and urine of patients receiving aphidicolin-17-glycinate. The major metabolite found in plasma was 3-ketoaphidicolin, present at about one thent the concentrations of aphidicolin. 3-Ketoaphidicolin undergoes dehydroxymethylation to give 18-nor-3-ketoaphidicolin. This metabolite was also found in plasma and its concentration reached a maximum of 1% of aphidicolin. Small amounts of aphidicolin and 3-ketoaphidicolin were found free in urine but almost all of the drug was conjugated to glucuronic acid, as shown by mass spectrometric analysis of urine extracts and by enzymatic digestion with β-glucoronidase followed by gas chromatographic/mass spectrometric analysis.

AB - We used gas chromatography/mass spectrometry to identify and quantitate some aphidicolin metabelites in plasma and urine of patients receiving aphidicolin-17-glycinate. The major metabolite found in plasma was 3-ketoaphidicolin, present at about one thent the concentrations of aphidicolin. 3-Ketoaphidicolin undergoes dehydroxymethylation to give 18-nor-3-ketoaphidicolin. This metabolite was also found in plasma and its concentration reached a maximum of 1% of aphidicolin. Small amounts of aphidicolin and 3-ketoaphidicolin were found free in urine but almost all of the drug was conjugated to glucuronic acid, as shown by mass spectrometric analysis of urine extracts and by enzymatic digestion with β-glucoronidase followed by gas chromatographic/mass spectrometric analysis.

UR - http://www.scopus.com/inward/record.url?scp=0027610340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027610340&partnerID=8YFLogxK

U2 - 10.1002/bms.1200220607

DO - 10.1002/bms.1200220607

M3 - Article

C2 - 8329465

AN - SCOPUS:0027610340

VL - 22

SP - 351

EP - 357

JO - Biological Mass Spectrometry

JF - Biological Mass Spectrometry

SN - 1052-9306

IS - 6

ER -