Mass spectrometric identification of hemoglobin modifications induced by nitrosobenzene

Francesco Di Girolamo, Luigi Campanella, Roberto Samperi, Angela Bachi

Research output: Contribution to journalArticlepeer-review

Abstract

Aniline and nitrobenzene (NB) are widely used industrial chemicals. Early effects of aniline toxicity include methemoglobin formation and damage to erythrocytes (Jenkins, F.P., 1972. The no-effect dose of anilne in human subjects and a comparison of aniline toxicity in man and rat. Food Cosmet. Toxicol. 10, 671-679; Bus, J.S., Popp, J.A., 1987. Perspectives on the mechanism of action of the splenic toxicity of aniline and structurally-related. Food Chem. Toxicol. 25, 619-627). In this report, we describe an analytical method, based on LC techniques and mass spectrometry, which could help in monitoring the exposure to aniline and NB. In particular, we describe and characterize the formation of specific adducts during an in vitro reaction of nitrosobenzene (NOB), the main metabolite of aniline and NB, and human hemoglobin.

Original languageEnglish
Pages (from-to)1601-1608
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume72
Issue number5
DOIs
Publication statusPublished - Jul 2009

Keywords

  • Aniline
  • Mass spectrometry
  • Nitrobenzene
  • Nitrosobenzene
  • Protein adduct

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Pollution

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