Mass spectrometry-based biomarker discovery

Weidong Zhou, Emanuel F. Petricoin, Caterina Longo

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Discovery of candidate biomarkers within the entire proteome is one of the most important and challenging goals in proteomic research. Mass spectrometry-based proteomic is a modern and promising technology for semiquantitative and qualitative assessment of proteins, enabling protein sequencing and identification with exquisite accuracy and sensitivity. For mass spectrometry analysis, protein extractions from tissues of interest or body fluids with subsequent protein fractionation represent an important and unavoidable step in the workflow for biomarker discovery. The aim of our chapter is to provide practical lab procedures for sample digestion and protein fractionation for subsequent mass spectrometry analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages251-264
Number of pages14
Volume823
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume823
ISSN (Print)10643745

Fingerprint

Mass Spectrometry
Biomarkers
Proteomics
Proteins
Workflow
Protein Sequence Analysis
Body Fluids
Proteome
Proteolysis
Technology
Research

Keywords

  • Biomarker discovery
  • In-gel digestion of proteins
  • In-solution digestion of proteins
  • LC-MS/MS
  • LTQ-Orbitrap
  • Mass spectrometry
  • SEQUEST

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zhou, W., Petricoin, E. F., & Longo, C. (2012). Mass spectrometry-based biomarker discovery. In Methods in Molecular Biology (Vol. 823, pp. 251-264). (Methods in Molecular Biology; Vol. 823). https://doi.org/10.1007/978-1-60327-216-2_16

Mass spectrometry-based biomarker discovery. / Zhou, Weidong; Petricoin, Emanuel F.; Longo, Caterina.

Methods in Molecular Biology. Vol. 823 2012. p. 251-264 (Methods in Molecular Biology; Vol. 823).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zhou, W, Petricoin, EF & Longo, C 2012, Mass spectrometry-based biomarker discovery. in Methods in Molecular Biology. vol. 823, Methods in Molecular Biology, vol. 823, pp. 251-264. https://doi.org/10.1007/978-1-60327-216-2_16
Zhou W, Petricoin EF, Longo C. Mass spectrometry-based biomarker discovery. In Methods in Molecular Biology. Vol. 823. 2012. p. 251-264. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-216-2_16
Zhou, Weidong ; Petricoin, Emanuel F. ; Longo, Caterina. / Mass spectrometry-based biomarker discovery. Methods in Molecular Biology. Vol. 823 2012. pp. 251-264 (Methods in Molecular Biology).
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