Mass spectrometry imaging reveals new biological roles for choline esters and Tyrian purple precursors in muricid molluscs

David Rudd, Maurizio Ronci, Martin R. Johnston, Taryn Guinan, Nicolas H. Voelcker, Kirsten Benkendorff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Despite significant advances in chemical ecology, the biodistribution, temporal changes and ecological function of most marine secondary metabolites remain unknown. One such example is the association between choline esters and Tyrian purple precursors in muricid molluscs. Mass spectrometry imaging (MSI) on nano-structured surfaces has emerged as a sophisticated platform for spatial analysis of low molecular mass metabolites in heterogeneous tissues, ideal for low abundant secondary metabolites. Here we applied desorption-ionisation on porous silicon (DIOS) to examine in situ changes in biodistribution over the reproductive cycle. DIOS-MSI showed muscle-relaxing choline ester murexine to co-localise with tyrindoxyl sulfate in the biosynthetic hypobranchial glands. But during egg-laying, murexine was transferred to the capsule gland, and then to the egg capsules, where chemical ripening resulted in Tyrian purple formation. Murexine was found to tranquilise the larvae and may relax the reproductive tract. This study shows that DIOS-MSI is a powerful tool that can provide new insights into marine chemo-ecology.

Original languageEnglish
Article number13408
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Sep 1 2015

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Molluscs
Porous silicon
Metabolites
Choline
Ionization
Mass spectrometry
Desorption
Esters
Ecology
Imaging techniques
Capsules
Molecular mass
Sulfates
Muscle
Association reactions
Tissue
6,6'-dibromoindigo
murexine

ASJC Scopus subject areas

  • General

Cite this

Mass spectrometry imaging reveals new biological roles for choline esters and Tyrian purple precursors in muricid molluscs. / Rudd, David; Ronci, Maurizio; Johnston, Martin R.; Guinan, Taryn; Voelcker, Nicolas H.; Benkendorff, Kirsten.

In: Scientific Reports, Vol. 5, 13408, 01.09.2015.

Research output: Contribution to journalArticle

Rudd, David ; Ronci, Maurizio ; Johnston, Martin R. ; Guinan, Taryn ; Voelcker, Nicolas H. ; Benkendorff, Kirsten. / Mass spectrometry imaging reveals new biological roles for choline esters and Tyrian purple precursors in muricid molluscs. In: Scientific Reports. 2015 ; Vol. 5.
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