Abstract
Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3 N 2 - 3 M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.
| Original language | English |
|---|---|
| Article number | 703163 |
| Journal | Gastroenterology Research and Practice |
| Volume | 2013 |
| DOIs | |
| Publication status | Published - 2013 |
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ASJC Scopus subject areas
- Gastroenterology
- Hepatology
Cite this
Mast cells positive to tryptase and c-kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated. / Ammendola, Michele; Sacco, Rosario; Sammarco, Giuseppe; Donato, Giuseppe; Zuccalà, Valeria; Romano, Roberto; Luposella, Maria; Patruno, Rosa; Vallicelli, Carlo; Verdecchia, Giorgio Maria; Cavaliere, Davide; Montemurro, Severino; Ranieri, Girolamo.
In: Gastroenterology Research and Practice, Vol. 2013, 703163, 2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mast cells positive to tryptase and c-kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated
AU - Ammendola, Michele
AU - Sacco, Rosario
AU - Sammarco, Giuseppe
AU - Donato, Giuseppe
AU - Zuccalà, Valeria
AU - Romano, Roberto
AU - Luposella, Maria
AU - Patruno, Rosa
AU - Vallicelli, Carlo
AU - Verdecchia, Giorgio Maria
AU - Cavaliere, Davide
AU - Montemurro, Severino
AU - Ranieri, Girolamo
PY - 2013
Y1 - 2013
N2 - Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3 N 2 - 3 M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.
AB - Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3 N 2 - 3 M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.
UR - http://www.scopus.com/inward/record.url?scp=84890032845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890032845&partnerID=8YFLogxK
U2 - 10.1155/2013/703163
DO - 10.1155/2013/703163
M3 - Article
AN - SCOPUS:84890032845
VL - 2013
JO - Gastroenterology Research and Practice
JF - Gastroenterology Research and Practice
SN - 1687-6121
M1 - 703163
ER -