Mast cells positive to tryptase and c-kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated

Michele Ammendola, Rosario Sacco, Giuseppe Sammarco, Giuseppe Donato, Valeria Zuccalà, Roberto Romano, Maria Luposella, Rosa Patruno, Carlo Vallicelli, Giorgio Maria Verdecchia, Davide Cavaliere, Severino Montemurro, Girolamo Ranieri

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3 N 2 - 3 M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.

Original languageEnglish
Article number703163
JournalGastroenterology Research and Practice
Volume2013
DOIs
Publication statusPublished - 2013

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Proto-Oncogene Proteins c-kit
Tryptases
Mast Cells
Stomach Neoplasms
Neoplasms
Gabexate
Peptide Hydrolases
Cell Count
Immunohistochemistry
Clinical Trials

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Mast cells positive to tryptase and c-kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated. / Ammendola, Michele; Sacco, Rosario; Sammarco, Giuseppe; Donato, Giuseppe; Zuccalà, Valeria; Romano, Roberto; Luposella, Maria; Patruno, Rosa; Vallicelli, Carlo; Verdecchia, Giorgio Maria; Cavaliere, Davide; Montemurro, Severino; Ranieri, Girolamo.

In: Gastroenterology Research and Practice, Vol. 2013, 703163, 2013.

Research output: Contribution to journalArticle

Ammendola, M, Sacco, R, Sammarco, G, Donato, G, Zuccalà, V, Romano, R, Luposella, M, Patruno, R, Vallicelli, C, Verdecchia, GM, Cavaliere, D, Montemurro, S & Ranieri, G 2013, 'Mast cells positive to tryptase and c-kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated', Gastroenterology Research and Practice, vol. 2013, 703163. https://doi.org/10.1155/2013/703163
Ammendola, Michele ; Sacco, Rosario ; Sammarco, Giuseppe ; Donato, Giuseppe ; Zuccalà, Valeria ; Romano, Roberto ; Luposella, Maria ; Patruno, Rosa ; Vallicelli, Carlo ; Verdecchia, Giorgio Maria ; Cavaliere, Davide ; Montemurro, Severino ; Ranieri, Girolamo. / Mast cells positive to tryptase and c-kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated. In: Gastroenterology Research and Practice. 2013 ; Vol. 2013.
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AU - Ammendola, Michele

AU - Sacco, Rosario

AU - Sammarco, Giuseppe

AU - Donato, Giuseppe

AU - Zuccalà, Valeria

AU - Romano, Roberto

AU - Luposella, Maria

AU - Patruno, Rosa

AU - Vallicelli, Carlo

AU - Verdecchia, Giorgio Maria

AU - Cavaliere, Davide

AU - Montemurro, Severino

AU - Ranieri, Girolamo

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N2 - Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3 N 2 - 3 M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.

AB - Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3 N 2 - 3 M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.

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