Mast cells positive to tryptase, endothelial cells positive to protease-activated receptor-2, and microvascular density correlate among themselves in hepatocellular carcinoma patients who have undergone surgery

Michele Ammendola, Rosario Sacco, Giuseppe Sammarco, Tullio Piardi, Valeria Zuccalà, Rosa Patruno, Alessandra Zullo, Nicola Zizzo, Bruno Nardo, Ilaria Marech, Alberto Crovace, Cosmo Damiano Gadaleta, Patrick Pessaux, Girolamo Ranieri

Research output: Contribution to journalArticle

Abstract

Background: Mast cells (MCs) can stimulate angiogenesis, releasing several proangiogenic cytokines stored in their cytoplasm. In particular MCs can release tryptase, a potent in vivo and in vitro proangiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase phosphorylation. Nevertheless, no data are available concerning the relationship between MC density positive to tryptase (MCDPT), endothelial cells positive to PAR-2 forming microvascular density (PAR-2-MVD), and classical MVD (C-MVD) in hepatocellular carcinoma (HCC) angiogenesis. This study analyzed the correlation between MCDPT, PAR-2-MVD, and C-MVD, each correlated to the others and to the main clinicopathological features, in early HCC patients who underwent surgery. Methods: A series of 53 HCC patients with early stage (stage 0 according to the Barcelona Clinic Liver Cancer Staging Classification) were selected and then underwent surgery. Tumor tissue samples were evaluated by means of immunohistochemistry and image analysis methods in terms of number of MCDPT, PAR-2-MVD, and C-MVD. Results: A significant correlation between MCDPT, PAR-2-MVD, and C-MVD groups, each correlated to the others, was found by Pearson t-test analysis (r ranged from 0.67 to 0.81; P-value ranged from 0.01 to 0.03). No other significant correlation was found. Conclusion: Our in vivo pilot data suggest that MCDPT and PAR-2-MVD may play a role in HCC angiogenesis and could be further evaluated as a target of antiangiogenic therapy.

Original languageEnglish
Pages (from-to)4465-4471
Number of pages7
JournalOncoTargets and Therapy
Volume9
DOIs
Publication statusPublished - Jul 21 2016

Keywords

  • Stromal cells
  • Translational research
  • Tumour angiogenesis

ASJC Scopus subject areas

  • Oncology
  • Pharmacology (medical)

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