Maternal and gestational factors and micronucleus frequencies in umbilical blood: The newgeneris rhea cohort in Crete

Kim van de Loock, Eleni Fthenou, Ilse Decordier, Georgia Chalkiadaki, Maria Keramarou, Gina Plas, Mathieu Roelants, Jos Kleinjans, Leda Chatzi, Franco Merlo, Manolis Kogevinas, Micheline Kirsch-Volders

Research output: Contribution to journalArticle

Abstract

Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes. Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother-child pairs. Demographic and lifestyle characteristics were collected. Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p <0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p <0.001). MNMONO frequencies in newborns were positively associated with the mother's body mass index and inversely associated with gestational age and mother's age, but we found no significant predictors of MNBN or CBPI in newborns. Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step.

Original languageEnglish
Pages (from-to)1460-1465
Number of pages6
JournalEnvironmental Health Perspectives
Volume119
Issue number10
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Rheiformes
Umbilicus
Greece
Mothers
Newborn Infant
Cytokinesis
T-Lymphocytes
Fetal Blood
Surrogate Mothers
Environmental Monitoring
Tumor Biomarkers
Cell Division
Gestational Age
Life Style
Body Mass Index
Demography

Keywords

  • Folate
  • Gestational age
  • Micronuclei
  • Mononucleated cells
  • Newborns
  • Vitamin b12

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

van de Loock, K., Fthenou, E., Decordier, I., Chalkiadaki, G., Keramarou, M., Plas, G., ... Kirsch-Volders, M. (2011). Maternal and gestational factors and micronucleus frequencies in umbilical blood: The newgeneris rhea cohort in Crete. Environmental Health Perspectives, 119(10), 1460-1465. https://doi.org/10.1289/ehp.1003246

Maternal and gestational factors and micronucleus frequencies in umbilical blood : The newgeneris rhea cohort in Crete. / van de Loock, Kim; Fthenou, Eleni; Decordier, Ilse; Chalkiadaki, Georgia; Keramarou, Maria; Plas, Gina; Roelants, Mathieu; Kleinjans, Jos; Chatzi, Leda; Merlo, Franco; Kogevinas, Manolis; Kirsch-Volders, Micheline.

In: Environmental Health Perspectives, Vol. 119, No. 10, 10.2011, p. 1460-1465.

Research output: Contribution to journalArticle

van de Loock, K, Fthenou, E, Decordier, I, Chalkiadaki, G, Keramarou, M, Plas, G, Roelants, M, Kleinjans, J, Chatzi, L, Merlo, F, Kogevinas, M & Kirsch-Volders, M 2011, 'Maternal and gestational factors and micronucleus frequencies in umbilical blood: The newgeneris rhea cohort in Crete', Environmental Health Perspectives, vol. 119, no. 10, pp. 1460-1465. https://doi.org/10.1289/ehp.1003246
van de Loock, Kim ; Fthenou, Eleni ; Decordier, Ilse ; Chalkiadaki, Georgia ; Keramarou, Maria ; Plas, Gina ; Roelants, Mathieu ; Kleinjans, Jos ; Chatzi, Leda ; Merlo, Franco ; Kogevinas, Manolis ; Kirsch-Volders, Micheline. / Maternal and gestational factors and micronucleus frequencies in umbilical blood : The newgeneris rhea cohort in Crete. In: Environmental Health Perspectives. 2011 ; Vol. 119, No. 10. pp. 1460-1465.
@article{9b59a35f64b14223a8632958a1800642,
title = "Maternal and gestational factors and micronucleus frequencies in umbilical blood: The newgeneris rhea cohort in Crete",
abstract = "Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes. Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother-child pairs. Demographic and lifestyle characteristics were collected. Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p <0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p <0.001). MNMONO frequencies in newborns were positively associated with the mother's body mass index and inversely associated with gestational age and mother's age, but we found no significant predictors of MNBN or CBPI in newborns. Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step.",
keywords = "Folate, Gestational age, Micronuclei, Mononucleated cells, Newborns, Vitamin b12",
author = "{van de Loock}, Kim and Eleni Fthenou and Ilse Decordier and Georgia Chalkiadaki and Maria Keramarou and Gina Plas and Mathieu Roelants and Jos Kleinjans and Leda Chatzi and Franco Merlo and Manolis Kogevinas and Micheline Kirsch-Volders",
year = "2011",
month = "10",
doi = "10.1289/ehp.1003246",
language = "English",
volume = "119",
pages = "1460--1465",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",
number = "10",

}

TY - JOUR

T1 - Maternal and gestational factors and micronucleus frequencies in umbilical blood

T2 - The newgeneris rhea cohort in Crete

AU - van de Loock, Kim

AU - Fthenou, Eleni

AU - Decordier, Ilse

AU - Chalkiadaki, Georgia

AU - Keramarou, Maria

AU - Plas, Gina

AU - Roelants, Mathieu

AU - Kleinjans, Jos

AU - Chatzi, Leda

AU - Merlo, Franco

AU - Kogevinas, Manolis

AU - Kirsch-Volders, Micheline

PY - 2011/10

Y1 - 2011/10

N2 - Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes. Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother-child pairs. Demographic and lifestyle characteristics were collected. Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p <0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p <0.001). MNMONO frequencies in newborns were positively associated with the mother's body mass index and inversely associated with gestational age and mother's age, but we found no significant predictors of MNBN or CBPI in newborns. Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step.

AB - Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes. Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother-child pairs. Demographic and lifestyle characteristics were collected. Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p <0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p <0.001). MNMONO frequencies in newborns were positively associated with the mother's body mass index and inversely associated with gestational age and mother's age, but we found no significant predictors of MNBN or CBPI in newborns. Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step.

KW - Folate

KW - Gestational age

KW - Micronuclei

KW - Mononucleated cells

KW - Newborns

KW - Vitamin b12

UR - http://www.scopus.com/inward/record.url?scp=80053436486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053436486&partnerID=8YFLogxK

U2 - 10.1289/ehp.1003246

DO - 10.1289/ehp.1003246

M3 - Article

C2 - 21622084

AN - SCOPUS:80053436486

VL - 119

SP - 1460

EP - 1465

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - 10

ER -