Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy

Francesca Colleoni, Debora Lattuada, Ambra Garretto, Maddalena Massari, Chiara Mand, Edgardo Somigliana, Irene Cetin

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Abstract

Objective: We investigated mitochondrial DNA (mtDNA) content in the maternal circulation of normal pregnancies of different gestational ages and in pregnancies complicated by intrauterine growth restriction (IUGR). Study Design: We examined 70 maternal blood samples: 13 nonpregnant women; 45 normal pregnancies, divided into the 3 trimesters; and 12 pregnancies complicated by IUGR. MtDNA content was determined by real-time quantitative polymerase chain reaction, using a genomic control and a target gene. Results: A highly significant progressive reduction in circulating mtDNA was observed in pregnant women of first, second, and third trimesters and compared to nonpregnant women (mean value: 237, 188, 144, and 283, respectively; P <.001). Moreover, mtDNA was significantly increased in women carrying IUGR fetuses compared to women with normal pregnancies (430 vs 144; P <.001). Conclusion: MtDNA could provide new insight into the mechanisms that occur during physiological gestation. Furthermore, mtDNA content may help recognize the IUGR disease in pregnancy.

Original languageEnglish
JournalAmerican Journal of Obstetrics and Gynecology
Volume203
Issue number4
DOIs
Publication statusPublished - Oct 2010

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Keywords

  • intrauterine growth restriction
  • maternal blood
  • mitochondrial DNA
  • pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Medicine(all)

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