Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring

Tomoya Yamashita, Stefan Freigang, Claudia Eberle, Jennifer Pattison, Sachin Gupta, Claudio Napoli, Wulf Palinski

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Maternal hypercholesterolemia during pregnancy increases offspring susceptibility to atherosclerosis by an oxidation-dependent mechanism. The present studies investigated whether maternal immunization with oxidized LDL (OxLDL) before pregnancy protects the fetus from atherogenic in utero programming by maternal hypercholesterolemia. Maternal immunization of NZW rabbits and LDL receptor-deficient mice indeed reduced atherosclerosis in adult offspring by up to 56%, but the protective effect could not be attributed to a reduction of fetal exposure to hypercholesterolemia alone, and even nonspecific immune stimulation with adjuvant only provided some protection. Unexpectedly, offspring of immunized mothers developed increased IgM antibodies to selective OxLDL epitopes and increased IgM-LDL immune complexes, compared with offspring of nonimmunized controls. Even naïve offspring of OxLDL-immunized mothers never exposed to postnatal hypercholesterolemia responded to a one-time OxLDL and KLH challenge with greater OxLDL-specific IgM responses, increased OxLDL-specific IgM-secreting B cells, and more IgM-LDL immune complexes. In contrast, maternal immunization with KLH, a T cell-dependent nonmammalian antigen, did not influence postnatal immune responses. Effects of maternal OxLDL-immunization on offspring B cells and selective antibodies were independent of transplacental passage of maternal immunoglobulins. Results show that maternal immunization with antigens prevalent in atherosclerotic lesions reduces atherogenesis in their offspring by mechanisms that include, but are not limited to, reduced fetal exposure to maternal hypercholesterolemia and lipid peroxidation. More importantly, they demonstrate in principle that maternal adaptive immunity to selective antigens influences postnatal B cell and antibody responses in offspring, and that modulation of in utero immune programming may influence immune-modulated diseases later in life.

Original languageEnglish
JournalCirculation Research
Volume99
Issue number7
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Immunization Programs
Atherosclerosis
Mothers
Hypercholesterolemia
Immunoglobulin M
Immunization
B-Lymphocytes
Antigen-Antibody Complex
Antigens
Multifetal Pregnancy Reduction
oxidized low density lipoprotein
Maternal Exposure
Pregnancy
Antibodies
LDL Receptors
Immune System Diseases
Adaptive Immunity
Lipid Peroxidation
Antibody Formation
Immunoglobulins

Keywords

  • Adaptive immunity
  • Arteriosclerosis
  • Developmental programming
  • Immunization
  • In utero programming
  • Oxidized LDL
  • Prevention

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring. / Yamashita, Tomoya; Freigang, Stefan; Eberle, Claudia; Pattison, Jennifer; Gupta, Sachin; Napoli, Claudio; Palinski, Wulf.

In: Circulation Research, Vol. 99, No. 7, 10.2006.

Research output: Contribution to journalArticle

Yamashita, Tomoya ; Freigang, Stefan ; Eberle, Claudia ; Pattison, Jennifer ; Gupta, Sachin ; Napoli, Claudio ; Palinski, Wulf. / Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring. In: Circulation Research. 2006 ; Vol. 99, No. 7.
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