Maternal smoking during pregnancy and DNA-methylation in children at age 5.5 years: Epigenome-wide-analysis in the European Childhood Obesity Project (CHOP)-study

Peter Rzehak, Richard Saffery, Eva Reischl, Marcela Covic, Simone Wahl, Veit Grote, Annick Xhonneux, Jean Paul Langhendries, Natalia Ferre, Ricardo Closa-Monasterolo, Enrica Riva, Piotr Socha, Dariusz Gruszfeld, Berthold Koletzko, Philippe Goyens, Clotilde Carlier, Joana Hoyos, Pascale Poncelet, Elena Dain, Jean Noel Van HeesFrançoise Martin, Joaquin Escribano, Veronica Luque, Georgina Mendez, Marta Zaragoza-Jordana, Marcello Giovannini, Carlo Agostoni, Silvia Scaglioni, Elvira Verduci, Fiammetta Vecchi, Alice Re Dionigi, Jerzy Socha, Anna Stolarczyk, Anna Dobrzanska, Roman Janas, Emmanuel Perrin, Rudiger Von Kries, Helfried Groebe, Anna Reith, Renate Hofmann, Martina Weber, Sonia Schiess, Jeannette Beyer, Michaela Fritsch, Uschi Handel, Ingrid Pawellek, Sabine Verwied-Jorky, Iris Hannibal, Hans Demmelmair, Gudrun Haile, Melissa Theurich

Research output: Contribution to journalArticlepeer-review

Abstract

Mounting evidence links prenatal exposure to maternal tobacco smoking with disruption of DNA methylation (DNAm) profile in the blood of infants. However, data on the postnatal stability of such DNAm signatures in childhood, as assessed by Epigenome Wide Association Studies (EWAS), are scarce. Objectives of this study were to investigate DNAm signatures associated with in utero tobacco smoke exposure beyond the 12th week of gestation in whole blood of children at age 5.5 years, to replicate previous findings in young European and American children and to assess their biological role by exploring databases and enrichment analysis. DNA methylation was measured in blood of 366 children of the multicentre European Childhood Obesity Project Study using the Illumina Infinium HM450 Beadchip (HM450K). An EWAS was conducted using linear regression of methylation values at each CpG site against in utero smoke exposure, adjusted for study characteristics, biological and technical effects. Methylation levels at five HM450K probes in MYO1G (cg12803068, cg22132788, cg19089201), CNTNAP2 (cg25949550), and FRMD4A (cg11813497) showed differential methylation that reached epigenome-wide significance according to the false-discovery-rate (FDR) criteria (q-value

Original languageEnglish
Article numbere0155554
JournalPLoS One
Volume11
Issue number5
DOIs
Publication statusPublished - May 1 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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