Mathematical modeling of the capillary-like pattern generated by adrenomedullin-treated human vascular endothelial cells in vitro

Diego Guidolin, Giovanna Albertin, Elisa Sorato, Barbara Oselladore, Alessandra Mascarin, Domenico Ribatti

Research output: Contribution to journalArticlepeer-review

Abstract

A recently proposed approach was used to model the self-organization into capillary-like structures of human vascular endothelial cells cultured on Matrigel. The model combines a Cellular Potts Model, considering cell adhesion, cytoskeletal rearrangement and chemotaxis, and a Partial Differential Equation model describing the release and the diffusion of a chemoattractant. The results were compared with the data from real in vitro experiments to establish the capability of the model to accurately reproduce both the spontaneous self-assembly of unstimulated cells and their self-organization in the presence of the proangiogenic factor adrenomedullin. The results showed that the model can accurately reproduce the self-assembly of unstimulated cells, but it failed in reproducing the adrenomedullin-induced self-organization of the cells. The extension of the model to include cell proliferation led to a good match between simulated and experimental patterns in both cases with predicted proliferation rates in agreement with the data of cell proliferation experiments.

Original languageEnglish
Pages (from-to)1951-1963
Number of pages13
JournalDevelopmental Dynamics
Volume238
Issue number8
DOIs
Publication statusPublished - Aug 2008

Keywords

  • Adrenomedullin
  • Angiogenesis
  • Cellular Potts model
  • Human vascular endothelial cells
  • Image analysis
  • Matrigel assay

ASJC Scopus subject areas

  • Developmental Biology

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