Matrix interferences in the analysis of benzene in urine

L. Perbellini, M. Buratti, M. L. Fiorentino, S. Fustinoni, F. Pasini, S. Magnaghi

Research output: Contribution to journalArticle

Abstract

The analysis of benzene in urine of the general population or of exposed workers can be performed with different methods using the 'purge and trap' or 'solid-phase microextraction' techniques in combination with gas chromatographic analysis and photoionisation or mass spectrometric detection. The published results, however, are deeply conflicting. Differences in sample preparation by different research groups and our own preliminary observations prompted us to investigate pre-analytical and analytical factors potentially capable of modifying the urinary benzene quantification results. Benzene concentrations were measured in 20 urine samples in relation to different conditioning conditions (at 24, 40 and 80°C) and at basic or acid pH. Urinary protein concentrations were measured in the same samples. Urine heating at 80°C yields benzene concentrations on average five times higher than at 24°C. On acidification of urine, the benzene released increases up to 28-fold in comparison to that obtained at uncorrected 'physiological' pH. Despite a widely scattered data distribution, a statistically significant linear correlation was found between 'heat-released' and 'acid-labile' benzene values. There was no correlation between total urinary proteins present in 'physiological' concentrations (between 12 and 110 mg/l) and the different kinds of benzene in urine. Our results could perhaps be explained if it is supposed that part of the benzene in urine is absorbed onto sediment, or bound to specific proteins, or derived from parent molecules and is released with pH modification or heat administration. Our observations may also help to explain why the urinary benzene concentrations reported by different investigators vary considerably even when environmental levels are comparable. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)257-264
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume724
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Benzene
Photoionization
Proteins
Acids
Acidification
Gas chromatography
Sediments
Heating
Molecules

Keywords

  • Benzene

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Matrix interferences in the analysis of benzene in urine. / Perbellini, L.; Buratti, M.; Fiorentino, M. L.; Fustinoni, S.; Pasini, F.; Magnaghi, S.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 724, No. 2, 1999, p. 257-264.

Research output: Contribution to journalArticle

@article{30830d39da0d4c3caab4363635ac1368,
title = "Matrix interferences in the analysis of benzene in urine",
abstract = "The analysis of benzene in urine of the general population or of exposed workers can be performed with different methods using the 'purge and trap' or 'solid-phase microextraction' techniques in combination with gas chromatographic analysis and photoionisation or mass spectrometric detection. The published results, however, are deeply conflicting. Differences in sample preparation by different research groups and our own preliminary observations prompted us to investigate pre-analytical and analytical factors potentially capable of modifying the urinary benzene quantification results. Benzene concentrations were measured in 20 urine samples in relation to different conditioning conditions (at 24, 40 and 80°C) and at basic or acid pH. Urinary protein concentrations were measured in the same samples. Urine heating at 80°C yields benzene concentrations on average five times higher than at 24°C. On acidification of urine, the benzene released increases up to 28-fold in comparison to that obtained at uncorrected 'physiological' pH. Despite a widely scattered data distribution, a statistically significant linear correlation was found between 'heat-released' and 'acid-labile' benzene values. There was no correlation between total urinary proteins present in 'physiological' concentrations (between 12 and 110 mg/l) and the different kinds of benzene in urine. Our results could perhaps be explained if it is supposed that part of the benzene in urine is absorbed onto sediment, or bound to specific proteins, or derived from parent molecules and is released with pH modification or heat administration. Our observations may also help to explain why the urinary benzene concentrations reported by different investigators vary considerably even when environmental levels are comparable. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "Benzene",
author = "L. Perbellini and M. Buratti and Fiorentino, {M. L.} and S. Fustinoni and F. Pasini and S. Magnaghi",
year = "1999",
doi = "10.1016/S0378-4347(98)00584-2",
language = "English",
volume = "724",
pages = "257--264",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "1387-2273",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Matrix interferences in the analysis of benzene in urine

AU - Perbellini, L.

AU - Buratti, M.

AU - Fiorentino, M. L.

AU - Fustinoni, S.

AU - Pasini, F.

AU - Magnaghi, S.

PY - 1999

Y1 - 1999

N2 - The analysis of benzene in urine of the general population or of exposed workers can be performed with different methods using the 'purge and trap' or 'solid-phase microextraction' techniques in combination with gas chromatographic analysis and photoionisation or mass spectrometric detection. The published results, however, are deeply conflicting. Differences in sample preparation by different research groups and our own preliminary observations prompted us to investigate pre-analytical and analytical factors potentially capable of modifying the urinary benzene quantification results. Benzene concentrations were measured in 20 urine samples in relation to different conditioning conditions (at 24, 40 and 80°C) and at basic or acid pH. Urinary protein concentrations were measured in the same samples. Urine heating at 80°C yields benzene concentrations on average five times higher than at 24°C. On acidification of urine, the benzene released increases up to 28-fold in comparison to that obtained at uncorrected 'physiological' pH. Despite a widely scattered data distribution, a statistically significant linear correlation was found between 'heat-released' and 'acid-labile' benzene values. There was no correlation between total urinary proteins present in 'physiological' concentrations (between 12 and 110 mg/l) and the different kinds of benzene in urine. Our results could perhaps be explained if it is supposed that part of the benzene in urine is absorbed onto sediment, or bound to specific proteins, or derived from parent molecules and is released with pH modification or heat administration. Our observations may also help to explain why the urinary benzene concentrations reported by different investigators vary considerably even when environmental levels are comparable. Copyright (C) 1999 Elsevier Science B.V.

AB - The analysis of benzene in urine of the general population or of exposed workers can be performed with different methods using the 'purge and trap' or 'solid-phase microextraction' techniques in combination with gas chromatographic analysis and photoionisation or mass spectrometric detection. The published results, however, are deeply conflicting. Differences in sample preparation by different research groups and our own preliminary observations prompted us to investigate pre-analytical and analytical factors potentially capable of modifying the urinary benzene quantification results. Benzene concentrations were measured in 20 urine samples in relation to different conditioning conditions (at 24, 40 and 80°C) and at basic or acid pH. Urinary protein concentrations were measured in the same samples. Urine heating at 80°C yields benzene concentrations on average five times higher than at 24°C. On acidification of urine, the benzene released increases up to 28-fold in comparison to that obtained at uncorrected 'physiological' pH. Despite a widely scattered data distribution, a statistically significant linear correlation was found between 'heat-released' and 'acid-labile' benzene values. There was no correlation between total urinary proteins present in 'physiological' concentrations (between 12 and 110 mg/l) and the different kinds of benzene in urine. Our results could perhaps be explained if it is supposed that part of the benzene in urine is absorbed onto sediment, or bound to specific proteins, or derived from parent molecules and is released with pH modification or heat administration. Our observations may also help to explain why the urinary benzene concentrations reported by different investigators vary considerably even when environmental levels are comparable. Copyright (C) 1999 Elsevier Science B.V.

KW - Benzene

UR - http://www.scopus.com/inward/record.url?scp=0033016562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033016562&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(98)00584-2

DO - 10.1016/S0378-4347(98)00584-2

M3 - Article

C2 - 10219666

AN - SCOPUS:0033016562

VL - 724

SP - 257

EP - 264

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 1387-2273

IS - 2

ER -